Skip to main content
SpringerLink
Log in

Intraspecific protoplast fusion of amylase-producing strains of Candida fennica

  • Short Communication
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The production of α-amylase was increased by protoplast fusion of auxotrophic mutants of Candida fennica FTPT-8903. One prototrophic fusant was 90% and 32% more efficient in producing α-amylase in semi-solid and liquid fermentation, respectively, than the parental strains. Protoplast fusion did not significantly stimulate the synthesis of glucoamylase in the fusants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Andrade, C.M.M., Oliveira, F.M. & Linardi, V.R. 1992 Candida fennica: enhancement of protoplast formation and fusion. Canadian Journal of Microbiology 38, 807–810.

    Google Scholar 

  • Dias, A.A.M., Andrade, C.M.M.C. & Linardi, V.R. 1992 Production of amylases by Candida in liquid and semisolid fermentation. Revista de Microbiologia 3, 189–193.

    Google Scholar 

  • Fujii, T., Yamamoto, H., Takenaka, E., Fujinami, K., Ando, A. & Yabuki, M. 1988 Intraspecific hybridization of a methanol utilizing yeast Candida sp. N-16 through protoplast fusion. Agricultural and Biological Chemistry 52, 1661–1667.

    Google Scholar 

  • Giles, K.W. & Myers, A. 1965 An improved diphenylamine method for the estimation of deoxyribonucleic acid. Nature 206, 93.

    PubMed  Google Scholar 

  • KregerVan Rij, N.J. 1984 The Yeasts, a Taxonomic Study, 3rd edn. Amsterdam: Elsevier Science.

    Google Scholar 

  • Poulter, R. & Hanrahan, V. 1983 Conservation of genetic linkage in nonisogenic isolates of Candida albicans. Journal of Bacteriology 156, 498–506.

    PubMed  Google Scholar 

  • Snedecor, G.W. & Cochran, W. G. 1967 Statistical Methods, 6th edn. Ames: Iowa State University.

    Google Scholar 

  • Wilson, J.J. & Ingledew, W.M. 1982 Isolation and characterization of Schwanniomyces alluvius amylolytic enzymes. Applied and Environmental Microbiology 44, 301–307.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Microbiologia, Universidade Federal de Minas Gerais, Cx.P.2486, 30161-970, Belo Horizonte, Brazil

    V. R. Linardi, C. M. M. C. Carvalho & A. A. M. Dias

Authors
  1. V. R. Linardi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. M. M. C. Carvalho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. A. M. Dias
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Linardi, V.R., Carvalho, C.M.M.C. & Dias, A.A.M. Intraspecific protoplast fusion of amylase-producing strains of Candida fennica . World J Microbiol Biotechnol 9, 601–602 (1993). https://doi.org/10.1007/BF00386305

Download citation

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00386305

Key words

Search

Navigation