Abstract
When the roots of rye plants grown at 20°C were cooled to 8°C the concentration of phospholipid in them more than doubled over a 7 d period in comparison with that in roots remaining at 20°C. The relative abundance of lecithin (PC) declined while that of phosphatidyl ethanolamine (PE) increased; this change was completed after 2 d cooling. Labelling with 32P suggested that turnover of phospholipids may be inhibited by low temperature. Acyl lipids contained an increased proportion of linolenic acid (18:3) and reduced proportion of linoleic acid (18:2) when roots were cooled at 8°C for 7 d. The ratio of these acids is a relatively more sensitive indicator of desaturation than is the double bond index. Cooling brought about no change in the abundance of the principal saturated acid, palmitic (16:0). In the first 3 days of cooling PC and PE desaturated markedly while there was no change in galactosyl and neutral lipids. Desaturation did not appear to be greatly sensitive to the concentration of dissolved O2 and was only partly inhibited in 8°C solutions where the oxygen concentration was lowered to 0.5–2.0%. Positional analysis of acyl chains in PC and PE showed that more than 90% of all 16:0 is associated with position I while 65% of the 18:2+18:3 is associated with position II. When roots are cooled the abundance of 18:3 increases in both chains but the relative distribution of saturated and unsaturated fatty acids remains constant in positions I and II. At both 20°C and 8°C there is a high probability that a saturated chain in position I will be paired with the polyunsaturated one in position II.
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Abbreviations
- PC:
-
Lecithin
- PE:
-
phosphatidyl ethanolamine
- TLC:
-
thin layerchromatography
- BHT:
-
butylatedhydroxytoluene
References
Chapman, D. (1975) Phase transitions and fluidity characteristics of lipids and cell membranes. Q. Rev. Biophys. 8, 185–235
Clarkson, D.T. (1976) The influence of temperature on the exudation of xylem sap from detached root systems of rye (Secale cereale) and barley (Hordeum vulgare). Planta 132, 297–304
Clarkson, D.T., Shone, M.G.T., Wood, A.V. (1974) The effect of pretreatment temperature on the exudation of xylem sap by detached barley root systems. Planta 121, 81–92
Cronan, J.E., Gelmann, E.P. (1975) Physical properties of membrane lipids: biological relevance and regulation. Bacteriol. Rev. 39, 232–256
Debuch, H. (1957) Über die enzymatische Spaltung des Lecithins aus Sojabohnen. Hoppe-Seyler's Z. Physiol. Chem. 306, 279–286
de la Roche, I.A., Andrews, C.J., Pomeroy, M.K., Weinberger, P., Kates, M. (1972) Lipid changes in winter wheat seedlings (Triticum aestivum L.) at temperatures inducing cold hardiness. Can. J. Bot. 50, 2401–2409
Devor, K.A., Mudd, J.B. (1973) Structural analysis of phosphatidylcholine of plant tissue. J. Lipid Res. 12, 396–402
Farkas, T., Déri-Hadlaczky, E., Belea, A. (1975) Effect of Temperature upon Linolenic acid level in wheat and rye seedlings. Lipids 10, 331–334
Folch, J., Lees, M., Sloane, S.G.H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509
Harris, P., James, A.T. (1969) The effect of low temperatures on fatty acid biosynthesis in plants. Biochem. J. 112, 325–330
James, R., Branton, D. (1973) Lipid and temperature-dependent structural changes in Acholeplasma laidlawii cell membranes. Biochim. Biophys. Acta 323, 378–390
Kedrowski, R.A., Chapin, F.S. III. (1978) Lipid properties of Carex aquatilis from hot spring and permafrost-dominated sites in Alaska: implications for nutrient requirements. Physiol. Plant. 44, 231–237
Keenan, R.W., Leonard, R.T., Hodges, T.K. (1973) Lipid composition of plasma membranes from Avena sativa roots. Cytobios 7, 103–112
Kimball, S.L., Salisbury, F.B. (1973) Ultrastructural changes of plants exposed to low temperatures. Am. J. Bot. 60, 1028–1033
Kuiper, P.J.C. (1964) Water uptake of higher plants as affected by root temperature. Meded. Landbouwhogesch., Wageningen 63–4, 1–11
Kuiper, P.J.C. (1970) Lipids in alfalfa leaves in relation to cold hardiness. Plant Physiol. 45, 684–686
Kuiper, P.J.C. (1974) Role of lipids in water and ion transport. In: Recent advances in chemistry and biochemistry of plant lipid. Proc. Phytochem. Soc. 12, 359–386
Lyons, J.M., Wheaton, T.A., Pratt, H.K. (1964) Relationship between the physical nature of mitochondrial membranes and chilling sensitivity in plants. Plant Physiol. 39, 262–268
Markhart, A.H. III, Fiscus, E.L., Naylor, A.W., Kramer, P.J. (1979) Effect of temperature on water and ion transport in soybean and broccoli systems. Plant Physiol. 64, 83–87
Martin, C.E., Hiramitsu, K., Kitajima, Y., Nozawa, Y., Skriver, L., Thompson, G.A., Jr. (1976) Molecular control of membrane properties during temperature acclimation. Fatty acid desaturase regulation of membrane fluidity in acclimating Tetrahymena cells. Biochemistry 15, 5218–5227
Mazliak, P. (1973) Lipid metabolism in plants. Annu. Rev. Plant Physiol. 24, 287–310
Mazliak, P. (1977) Glyco- and phospholipids of biomembranes in higher plants. In: Lipids and lipid polymers in higher plants, pp. 48–74 Tevini, M., Lichtenthaler, H.K., eds., Springer, Berlin Heidelberg New York
Miller, N.G.A., Hill, M.W., Smith, M.W. (1976) Positional and species analysis of membrane phospholipids extracted from goldfish adapted to different environmental temperatures. Biochim. Biophys. Acta 455, 644–654
Moreau, F., Dupont, J., Lance, C. (1974) Phospholipid and fatty acid composition of outer and inner membranes of plant mitochondria. Biochim. Biophys. Acta 345, 294–304
Mudd, J.B., van Vliet, H.H., van Deenan, L.L. (1969) Biosynthesis of galactolipids by enzyme preparations from spinach leaves. J. Lipid Res. 10, 623–630
Pomeroy, M.K., Andrews, C.J. (1978) Metabolic and ultrastructural changes in winter wheat during ice encasement under field conditions. Plant Physiol. 61, 806–811
Quail, P.H. (1979) Plant cell fractionation. Annu. Rev. Plant Physiol. 30, 425–484
Raison, J.K. (1973) Temperature-induced phase changes in membrane lipids and their influence on metabolic regulation. Symp. Soc. Exp. Biol. 27, 485
Raison, J.K., Chapman, E.A. (1976) Membrane phase changes in chilling-sensitive Vigna radiata and their significance to growth. Aust. J. Plant Physiol. 3, 291–299
Redshaw, E.S., Zalik, S. (1968) Changes in Lipids of cereal seedlings during vernalization. Can. J. Biochem. 46, 1093–1097
Simon, E.W. (1974) Phospholipids and Plant Membrane Permeability. New Phytol. 73, 377–420
Smolenska, G., Kuiper, P.J.C. (1977) Effect of low temperature upon lipid and fatty acid composition of roots and leaves of winter rape. Physiol. Plant. 41, 29–35
Stumpf, P.K. (1976) Lipid metabolism. In: Plant biochemistry, 3rd ed., pp. 427–461, Bonner, J., Varner, J.E. eds. Academic Press, New York
Vartapetian, B.B., Mazliak, P., Lance, C. (1978) Lipid biosynthesis in rice coleoptiles grown in the presence or in the absence of oxygen. Plant Sci. Lett. 13, 321–328
Wijngaarden, D. van (1967) Modified rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal. Chem 39, 848–849
Willemot, C. (1975) Stimulation of phospholipid biosynthesis during frost hardening of winter wheat. Plant Physiol. 55, 356–359
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Clarkson, D.T., Hall, K.C. & Roberts, J.K.M. Phospholipid composition and fatty acid desaturation in the roots of rye during acclimatization of low temperature. Planta 149, 464–471 (1980). https://doi.org/10.1007/BF00385749
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DOI: https://doi.org/10.1007/BF00385749