Abstract
Arginine transport in suspension-cultured cells of Nicotiana tabacum L. cv. Wisconsin-38 was investigated. Cells that were preincubated in the presence of Ca2+ for 6 h prior to transport exhibited stimulated transport rates. After the preincubation treatment, initial rates of uptake were constant for at least 45 min. Arginine accumulated in the cells against a concentration gradient; this accumulation was not the result of exchange diffusion. Arginine uptake over a concentration range of 2.5 μM to 1 mM was characterized by simple Michaelis-Menten kinetics with a Km of 0.1 mM and a Vmax of 9,000 nmol g-1 fresh weight h-1. Transport was inhibited by several compounds including carbonylcyanide-m-chlorophenylhydrazone, 2,4-dinitrophenol, N,N′-dicyclohexylcarbodiimide, and N-ethylmaleimide. Inhibition by these compounds was not the result of increased efflux resulting from membrane damage. A variety of amino acids and analogs, with the exception of D-arginine, inhibited transport, indicating that arginine transport was mediated by a general L-aminoacid permease. Competition experiments indicated that arginine and lysine exhibited cross-competition for transport, with Ki values similar to respective Km values. Arginine transport and low-affinity lysine transport are probably mediated by the same system in these cells.
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Abbreviations
- BTP:
-
Bis Tris Propane
- CCCP:
-
Carbonylcyanide-m-chlorophenylhydrazone
- DCCD:
-
N,N′-dicyclohexylcarbodiimide
- DNP:
-
2,4-dinitrophenol
- DTT:
-
Dithiothreitol
- NEM:
-
N-ethylmaleimide
- MES:
-
2(N-morpholino)ethanesulfonic acid
- TCA:
-
trichloroacetic acid
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This paper is the third in a series on amino-acid transport into cultured tobacco cells. For parts I and II, see Harrington and Henke (1981) and Harrington et al. (1981)
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Berry, S.L., Harrington, H.M., Bernstein, R.L. et al. Amino-acid transport into cultured tobacco cells. Planta 153, 511–518 (1981). https://doi.org/10.1007/BF00385534
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DOI: https://doi.org/10.1007/BF00385534