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Photosynthetic apparatus in chilling-sensitive plants

III. Contribution of loosely bound manganese to the mechanism of reversible inactivation of hill reaction activity following cold and dark storage and illumination of Leaves

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Abstract

The levels of both tightly and loosely bound Mn in chloroplasts from fresh, cold and dark stored as well as illuminated leaves of Lycopersicon esculentum Mill. were studied in relation to Hill reaction activity. The tightly bound Mn pool represents one third of the total Mn content in chloroplasts isolated from the fresh leaves, and its level does not change following cold storage and illumination of leaves. Upon cold and dark storage of leaves the loss from the chloroplasts of about 40%–50% of the total amount of Mn is accompanied by an almost complete inactivation of the Hill reactions, as studied with water as an electron donor, as well as by the appearance of an EPR signal characteristic of free Mn2+ ions. Following illumination of such leaves, the restroration of Hill reaction activity is accompanied by an increase in the total Mn content in chloroplasts of up to 70%–80% of the Mn level measured in the fresh leaves and by disappearance of the EPR signal. In contrast, aging in the cold of isolated chloroplasts does not affect their Mn content. The addition of manganese does not result in the restoration of Hill reaction activity in chloroplasts from cold stored leaves but causes a restoration of this activity inhibited by linolenic acid. The data suggest that the loosely bound Mn pool (extractable with Tris) can be differentiated into two fractions: (1) one functionally inactive in electron transport and (2) one responsible for restoration of Hill reaction activity. Mn of the latter fraction (about 45% of the total Mn content) probably originates from the free Mn ions present in the interior of the chloroplasts following the cold and dark storage of leaves and from Mn reincorporated into chloroplasts from the cytoplasm. Incorporation of Mn from both these sources into thylakoid membrane to form a functionally active, loosely bound Mn pool proceeds during the illumination of leaves and results in the restoration of Hill reaction activity inhibited following the storage of leaves in dark and cold.

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Abbreviations

Chl:

chlorophyll

DCIP:

2,6-dichlorophenolindophenol

Diquat:

1,1-ethylene 2,2′-dipiridylium dibromide

EPR:

electron paramagnetic resonance

FFA:

free fatty acid

MV:

methyl viologen, N,N′-dimethyl-4,4′ dipyridyldihydrochloride

Tris:

tris-(hydroxymethyl) aminomethane

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Author notes

  1. J. ZĄbek

    Present address: Institute of Rheumatology, Spartańska I, PL 02-637, Warszawa, Poland

Authors and Affiliations

  1. Institute of Biochemistry, University of Warsaw, Al. Zwirki i Wigury 93, PL 02-089, Warszawa, Poland

    Z. Kaniuga, J. ZĄbek & Barbara Sochanowicz

Authors
  1. Z. Kaniuga
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  2. J. ZĄbek
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  3. Barbara Sochanowicz
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Kaniuga, Z., ZĄbek, J. & Sochanowicz, B. Photosynthetic apparatus in chilling-sensitive plants. Planta 144, 49–56 (1978). https://doi.org/10.1007/BF00385006

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  • DOI: https://doi.org/10.1007/BF00385006

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