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Mutant of Escherichia coli with unusual patterns of rpoB,C expression in response to rifampicin and acridine orange

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Summary

A mutation located near rpoB (89′) in E. coli is responsible for unusual patterns of β and β′ (but not L7/L12) synthesis in response to the drugs rifampicin and acridine orange.

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References

  • Barry G, Squires C, Squires CL (1980) Attenuation and processing of RNA from the rplJL-rpoBC transcription unit of Escherichia coli. Proc Natl Acad Sci USA 77:3331–3335

    Article  PubMed  CAS  Google Scholar 

  • Barry G, Squires CL, Squires C (1979) Control features within the rplJL-rpoBC transcription unit of Escherichia coli. Proc Natl Acad Sci USA 76:4922–4926

    Article  PubMed  CAS  Google Scholar 

  • Blumenthal RM, Dennis PP (1978) Gene expression in Escherichia coli B/r during partial rifampicin-mediated restriction of transcription initiation. Mol Gen Genet 165:79–86

    Article  PubMed  CAS  Google Scholar 

  • Chao L (1977) Regulation of RNA polymerase subunit synthesis in Escherichia coli: Utilization of DNA-intercalating drugs as a probe. Arch Biochem Biophys 183:242–249

    Article  PubMed  CAS  Google Scholar 

  • Dennis PP (1977) Regulation of synthesis and activity of a mutant RNA polymerase in Escherichia coli. Proc Natl Acad Sci USA 74:5116–5420

    Article  Google Scholar 

  • Fiil NP, Friesen JD, Downing W, Dennis PP (1980) Post-transcriptional regulatory mutants in a ribosomal protein-RNA polymerase operon of E. coli. Cell 19:837–844

    Article  PubMed  CAS  Google Scholar 

  • Fukuda R, Taketo M, Ishihama A (1978) Autogenous regulation of RNA polymerase beta subunit synthesis in vitro. J Biol Chem 253:4501–4504

    PubMed  CAS  Google Scholar 

  • Gershch NF, Jordan DO (1965) Interaction of DNA with aminoacridines. J Mol Biol 13:138–156

    Article  Google Scholar 

  • Hayward RS, Fyfe SK (1978) Non-coordinate expression of the neighbouring genes rplL (L7/L12) and rpoB,C of Escherichia coli. Mol Gen Genet 160:77–80

    PubMed  CAS  Google Scholar 

  • Hayward RS, Tittawella IPB, Scaife JG (1973) Evidence for specific control of RNA polymerase synthesis in Escherichia coli. Nature New Biol (London) 243:6–9

    CAS  Google Scholar 

  • Li K, Subramanian A (1975) Selective separation procedure for determination of ribosomal protein L7 and L12. Anal Biochem 64:121–129

    Article  PubMed  CAS  Google Scholar 

  • Lindahl L, Yamamoto M, Nomura M, Kirschbaum JB, Allet B, Rochaix J.-D (1977) Mapping of a cluster of genes for components of the transcriptional and translational mechanisms of Escherichia coli. J Mol Biol 109:23–47

    Article  PubMed  CAS  Google Scholar 

  • Linn T, Scaife J (1978) Identification of a single promoter in Escherichia coli for rplJ, rplL and rpoB,C. Nature (London) 276:33–37

    Article  CAS  Google Scholar 

  • Little R, Fiil NP, Dennis PP (1981) Transcriptional and post-transcriptional control of ribosomal protein and ribonucleic acid polymerase genes. J Bacteriol 147:25–35

    PubMed  CAS  Google Scholar 

  • Ma J-C, Newman AJ, Hayward RS (1981) Internal promoters of the rpoBC operon of Escherichia coli. Mol Gen Genet 184:548–550

    Article  PubMed  CAS  Google Scholar 

  • Newman A, Linn T, Hayward R (1979) Evidence for cotranscription of the RNA polymerase genes rpoBC with a ribosomal protein gene of Escherichia coli. Mol Gen Genet 169:195–204

    Article  PubMed  CAS  Google Scholar 

  • Post LE, Strycharz GD, Nomura M, Lewis H, Dennis PP (1979) Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in E. coli. Proc Natl Acad Sci USA 76:1697–1701

    Article  PubMed  CAS  Google Scholar 

  • Ramgopal S, Subramanian AR (1974) Alteration in the acetylation level of ribosomal protein L12 during growth cycle of Escherichia coli. Proc Natl Acad Sci USA 71:2136–2140

    Article  Google Scholar 

  • Scaife (1975) Bacterial RNA polymerases: the genetics and control of their synthesis. In: Losick R, Chamberlin M (eds), RNA polymerase. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 207–225

    Google Scholar 

  • Tittawella IPB (1976) Rifampicin-induced protein synthesis: A prerequisite for increased expression of the ββ′ operon in Escherichia coli. Mol Gen Genet 146:79–83

    Article  PubMed  CAS  Google Scholar 

  • Tittawella IPB (1981) Escherichia coli merodiploids with altered ratios of episomal to chromosomal RNA polymerase activity. Mol Gen Genet 184:504–507

    Article  PubMed  CAS  Google Scholar 

  • Tittawella IPB, Hayward RS (1974) Different effects of rifampicin and streptolydigin on the control of RNA polymerase subunit synthesis in Escherichia coli. Mol Gen Genet 134:181–186

    Article  PubMed  CAS  Google Scholar 

  • Waring MJ (1965) The effects of antimicrobial agents on ribonucleic acid polymerase. Mol Pharmacol 1:1–13

    PubMed  CAS  Google Scholar 

  • Yamamoto M, Nomura M (1978) Co-transcription of genes for RNA polymerase subunits beta and beta-prime with genes for ribosomal proteins in Escherichia coli. Proc Natl Acad Sci USA 75:3891–3895

    Article  PubMed  CAS  Google Scholar 

  • Yamamoto M, Nomura M (1979) Organization of the genes for transcription and translation in the rif region of the Escherichia coli chromosome. J Bacteriol 137:584–594

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, University of Umeå, S-90187, Umaå, Sweden

    Ivor P. B. Tittawella

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  1. Ivor P. B. Tittawella
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Comunicated by E. Bautz

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Tittawella, I.P.B. Mutant of Escherichia coli with unusual patterns of rpoB,C expression in response to rifampicin and acridine orange. Molec. Gen. Genet. 200, 114–117 (1985). https://doi.org/10.1007/BF00383322

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  • DOI: https://doi.org/10.1007/BF00383322

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