Summary
The majority of DNA lesions resulting from interactions of carcinogens with DNA are usually either single strand breaks or lesions which are converted to single strand breaks by treatment of DNA with alkaline solutions. A sensitive method of detecting DNA single strand breaks is the alkaline filter elution of DNA. We started to test this method for biomonitoring occupational exposure with sensitive experimental conditions using pH 12.6, where most alkali-labile DNA lesions are converted to single strand breaks. Under our conditions statistically significant differences can be detected between the elution rates of untreated V79 cells and cells treated with [3H]-thymidine 24 h prior to the elution. Statistically significant increases were detected in the elution rates of male smoking automobile mechanics and male smoking painters compared to non-smoking controls. No statistically significant differences were detected in the elution rates of male non-smoking automobile mechanics and male workers with a suspected exposure to halogenated aromatics compared to male controls. No statistically significant differences were observed in the elution rates of female smoking dry-cleaning workers compared to female smoking controls. Our experience showed that the alkaline elution technique can be a valuable tool for monitoring DNA damage in peripheral lymphocytes in man.
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References
Bermudez E, Mirsalis JC, Eales HC (1982) Detection of DNA damage in primary cultures of rat hepatocytes following in vivo and in vitro exposure to genotoxic agents. Environ Mutagen 4:667–679
Cantoni O, Costa M (1984) Analysis of the induction of alkali sensitive sites in the DNA by chromate and other agents that induce single strand breaks. Carcinogenesis 5:1207–1209
Doerjer G, Bedell MA, Oesch F (1984) DNA adducts and their biological relevance. In: Obe G (ed) Mutations in man. Springer, Berlin, pp 20–34
Glatt HR, Kaltenbach E, Oesch F (1980) Epoxide hydrolase activity in native and in mitogen-stimulated lymphocytes of various human donors. Cancer Res 40:2552–2556
Kohn KW, Ewing RAG, Erickson LC, Zwelling LA (1980) Measurements of strand breaks and crosslinks in DNA by alkaline elution. In: Friedberg EC, Hanawalt PC (eds) DNA repair. Marcel Dekker, New York, pp 379–401
Larsen KH, Brash D, Cleaver JE, Hart RW, Maher VM, Painter RB, Sega GA (1982) DNA repair assays as tests for environmental mutagens. A report of the U.S. EPA Gene-Tox Program. Mutat Res 98:287–318
Oesch F, Aulmann W, Platt KL, Doerjer G (1986) Individual differences in repair capacities in man. Arch Toxicol 1986 [Suppl 10] 172–179
Sina JF, Bean CL, Dysart GR, Taylor VI, Bradley MO (1983) Evaluation of the alkaline elution/rat hepatocyte assay as a predictor of carcinogenic/mutagenic potential. Mutat Res 113:357–391
Skare JA, Schrotel KR (1984) Validation of an in vivo alkaline elution assay to detect DNA damage in rat testicular cells. Environ Mutagen 7:563–576
Stout DL, Becker FF (1982) Fluorometric quantitation of single-stranded DNA: a method applicable to the technique of alkaline elution. Anal Biochem 127:302–307
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Supported by the Bundesministerium fur Forschung und Technologie grant 01HK354 0
This study contains part of an M.D. Thesis
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Doerjer, G., Buchholz, U., Kreuzer, K. et al. Biomonitoring of DNA damage by alkaline filter elution. Int Arch Occup Environ Health 60, 169–174 (1988). https://doi.org/10.1007/BF00378693
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DOI: https://doi.org/10.1007/BF00378693