Abstract
A panel of 63 radiation-reduced hybrids has been derived from a mouse cell line containing a neo-marked human Chromosome (Chr) 6, primarily to provide a resource for higher resolution localization of new markers. Hybrids were generated with radiation doses of 40–400 Gy, selected in G418, and were shown by PCR to contain the neo gene. PCR was also used to score the retention of 15 loci that map from 6q13 to q25.2 of the current consensus map plus six other loci assigned to 6q26-q27. An average retention frequency of 27.8% was observed, with the highest frequencies at D6S313 and D6S280 (63.5%) located near the centromere at 6q13, and at D6S283 (68.5%) at 6q16.3-q21, presumably close to the neo integration site. Lowest frequencies (4.8%) were observed for telomeric markers. All markers segregated independently except D6S297 and D6S193. Agreement and some improvement to the current consensus map of 6q was made by mapping 12 loci by the non-parametric statistical method of Falk. In addition, deletion mapping with informative hybrids allowed the ordering of six loci from 6q26 to q27 and permitted some integration of maps of this region.
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Boehnke, M., Lange, K., Cox, D.R. (1991). Statistical methods for multipoint radiation hybrid mapping. Am. J. Hum. Genet. 49, 1174–1188.
Boyle, J.M., Hey, Y., Myers, K., Stern, P.L., Grzeschik, F.-H., Ikehara, Y., Misumi, Y., Fox, M. (1992). Regional localization of a trophoblast antigen-related sequence and 16 other sequences to human chromosome 6q using somatic cell hybrids. Genomics 12, 693–698.
Cox, D.R., Burmeister, M., Price, E.R., Kim, S., Myers, R.M. (1990). Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes. Science 250, 245–250.
Devilee, P., Vliet, M.V., Sloun, P.v., Dijkshoorn, N.K., Hermans, J., Pearson, P.L., Cornelisse, L.J. (1991). Allelotype of human breast carcinoma: a second major site for loss of heterozygosity is on chromosome 6q. Oncogene 6, 1705–1711.
Doucette-Stamm, L.A., Riba, L., Handelin, B., Difilippantonio, M., Ward, D.C., Wasmuth, J.J., Gusella, J.F., Housman, D.E. (1991). Generation and characterisation of irradiation hybrids of human chromosome 4. Somatic Cell Mol. Genet. 17, 471–480.
Falk, C.T. (1991). A simple method for ordering loci using data from radiation hybrids. Genomics 9, 120–123.
Galfre, G., Howe, S.C., Milstein, C., Butcher, G.W., Howard, J.C. (1977). Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266, 550–552.
Hubbard-Smith, K., Patsalis, P., Pardinas, J.R., Jha, K.K., Henderson, A.S., Ozer, H.L. (1992). Altered human chromosome 6 in immortalised human fibroblasts. Mol. Cell. Biol. 12, 2273–2281.
Kievits, T., Devilee, P., Wiegant, J., Wapenaar, M.C., Cornelisse, C.J., van Ommen, G.J.B., Pearson, P.L. (1990). Direct nonradioactive in situ hybridisation of somatic cell hybrid DNA to human lymphocyte chromosomes. Cytometry 11, 105–109.
Lee, J.H., Kavanagh, J.J., Wildrick, D.M., Wharton, J.T., Blick, M. (1990). Frequent loss of heterozygosity on chromosome 6q11-17 in human ovarian carcinoma. Cancer Res. 50, 2724–2728.
Majello, B., Kenyon, L.C., Dalla-Favera, R. (1986). Human c-myb protooncogene: nucleotide sequence of cDNA and organsation of the genomic locus. Proc. Natl. Acad. Sci. USA 83, 9636–9640.
Malinowski, D.P., Sadler, E.J., Davie, E.W. (1984). Characterisation of a complementary deoxyribonucleic acid coding for human and bovine plasminogen. Biochemistry 23, 4243–4250.
Meese, E.U., Witkowski, C.M., Zoghbi, H.Y., Stanbridge, E.J., Meltzer, P.S., Trent, J.M. (1992). Development and utilisation of a somatic cell hybrid mapping panel to assign NotI linking probes to the long arm of human chromosome 6. Genomics 12, 542–548.
Meloni, R., Fougerousse, F., Roudaut, C., Beckman, J.S. (1992). Dinucleotide repeat polymorphism at the human liver arginase gene (ARG1). Nucleic Acids Res. 20, 1166.
Menasce, L.P., Orphanos, V., Santibanez-Koref, M.S., Boyle, J.M., Harrison, C.J. (1994a). Common region of deletion of the long arm of chromosome 6 in Non-Hodgkin's lymphoma and acute lymphoblastic leukemia. Genes Chromosome Cancer 10, 286–288.
Menasce, L.P., White, G.R.M., Harrison, C.J., Boyle, J.M., (1994b). Deletion of a common region on the long arm of chromosome 6 in acute lymphoblastic leukemia. Genes Chromosome. Cancer 10, 26–29.
Mittelman, F. (1991). Catalog of Chromosome Aberrations in Cancer, 4th ed. (New York: Wiley-Liss).
Nelson, D.L., Ledbetter, S.A., Corbo, L., Victoria, M.F., Ramirez-Solis, R., Webster, T.D., Ledbetter, D.H., Caskey, C.T. (1989). Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources. Proc. Natl. Acad. Sci. USA 86, 6686–6690.
Orphanos, V., Santibanez-Koref, M., McGown, G., Rackstraw, C., Boyle, J.M. (1994). Physical mapping of 43 STSs to human chromosome 6. Genomics 20, 301–304.
Ragoussis, J., Jones, T.A., Sheer, D., Shrimpton, A.E., Goodfellow, P.N., Trowsdale, J., Ziegler, A. (1991). Isolation of probes specific to human chromosomal region 6p21 from immunoselected irradiation-fusion gene transfer hybrids. Genomics 10, 598–607.
Rao, P.H., Murty, V.V.V.S., Gaidano, G., Haupstein, R., Dalla-Favera, R., Chaganti, R.S.K. (1994). Subregional localisation of 8 single copy loci to chromosome 6 by fluoresence in situ hybridisation. Cytogenet. Cell Genet. 66, 272–273.
Saito, S., Okui, K., Tokino, T., Oshimura, M., Nakamura, Y. (1992). Isolation and mapping of 68 RFLP markers on human chromosome 6. Am. J. Hum. Genet. 50, 65–70.
Shortle, V.P., Malaspina, P., Fox, M., Dooley, J., Volz, A., Ziegler, A., Trowsdale, J., Morrison, K., Edwards, Y.H. (1993). A cosmid library specific for human chromosome regions 6p21.3 and 6q27. Mamm. Genome 4, 493–498.
Siden, T.S., Kumlein, J., Schwartz, C.E., Rohme, D. (1992). Radiation fusion hybrids for chromosomes 3 and X generated at various irradiation doses. Somatic Cell Mol. Genet. 18, 33–44.
St. Clair, D.K., Holland, J.C. (1991). Complementary DNA encoding human colon cancer manganese superoxide dismutase and the expression of its gene in human cells. Cancer Res. 51, 939–943.
Tamari, M., Hamaguchi, M., Shimizu, M., Oshimura, M., m Takayama, H., Kohno, T., Yamaguchi, N., Sugimura, T., Terada, M., Yokoto, J. (1992). Ordering of human chromosome 3p markers by radiation hybrid mapping. Genomics 13, 705–712.
Trent, J.M., Stanbridge, E.J., McBride, H.L., Meese, E.U., Casey, G., Araujo, D.E., Witkowski, C.M., Nagle, R.B. (1990). Tumourigenicity in human melanoma cell lines controlled by introduction of human chromosome 6. Science 247, 568–571.
Volz, A., Boyle, J.M., Cann, H.M., Cottingham, R.W., Orr, H.T., Ziegler, A. (1994). Report of the 2nd International Workshop on Human Chromosome 6. Genomics 21, 464–472.
Weber, J.L., Kwitek, A.E., May, P.E. (1990). Dinucleotide repeat polymorphism at the D6S87 locus. Nucleic Acids Res. 18, 4636.
Weissenbach, J., Gyapay, G., Dib, C., Vignal, A., Morissette, J., Millasseau, P., Vaysseix, G., Lathrop, M. (1992). A second-generation linkage map of the human genome. Nature 359, 794–801.
Zoghbi, H.Y., McCall, A.E., LeBorgne-Demarquoy, F. (1991). Sixty-five radiation hybrids for the short arm of human chromosome 6: their value as a mapping panel and as a source for rapid isolation of new probes using repeat element-mediated PCR. Genomics 9, 713–720.
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Orphanos, V., Greaves, M., Santibanez-Koref, M. et al. A radiation hybrid panel for human Chromosome 6q. Mammalian Genome 6, 285–290 (1995). https://doi.org/10.1007/BF00352418
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DOI: https://doi.org/10.1007/BF00352418