Abstract
As a collaborative work of three laboratories the polymorphism of the canine fourth complement component (C4) was studied in a total of 131 unrelated dogs from different breeds and mongrels. Using high voltage electrophoresis followed by an immunoblotting technique, we detected eight distinct variants. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of canine C4 showed an additional heterogeneity of the α and γ chains which resulted in a total of 11 variants in the population studied. So that more precise information concerning the respective C4 allotypes will be available, a nomenclature is proposed designating not only the migration pattern of the C4 variants in agarose gels but also the heterogeneity of the C4 chains observed in SDS-PAGE.
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Abbreviations
- AGE:
-
agarose gel electrophoresis
- C4:
-
fourth complement component
- DLA:
-
dog leukocyte antigen
- EDTA:
-
ethylene diaminetetraacetate
- FCS:
-
fetal calf serum
- PBS:
-
phosphate-buffered saline
- PBST:
-
PBS and 0.22% Tween-20
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
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Doxiadis, G., Schoen, W., Doxiadis, I. et al. Statement on the nomenclature of dog C4 allotypes. Immunogenetics 25, 167–170 (1987). https://doi.org/10.1007/BF00344030
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DOI: https://doi.org/10.1007/BF00344030