Abstract
The B10.STA62 strain carries the H-2 w27 haplotype derived from a wild mouse captured in the vicinity of Ann Arbor, Michigan. Products of two class II loci composing this haplotype, A α and A β, are serologically, biochemically (by tryptic peptide mapping), and functionally indistinguishable from products controlled by the A bα and A /b β genes of the B10.A(5R) strain. In contrast, the polypeptide chain controlled by the third class II locus, E β, is different from that controlled by the E /b β gene. This E /w27 β chain lacks an antigenic determinant present on the Eb molecule and carries determinants lacking on the Eb molecule, the E /b β and E /w27 β peptide maps differ in at least six peptides, and cytotoxic T cells specific for the E b β chains do not react with B10.STA62 target cells. This great difference between the E /b β and E /w27 β chains suggests that the corresponding genes have not been derived from one another by a direct mutational conversion; instead, H-2w27 appears to be a recombinant haplotype derived by crossing-over between the A α A βduplex and the E βlocus. This is the first recombinant discovered separating these class II loci.
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Singh, S.K., Wakeland, E.K., Vučak, I. et al. An H-2 haplotype possibly derived by crossing-over between the (A α A β ) duplex and the E βlocus. Immunogenetics 14, 273–281 (1981). https://doi.org/10.1007/BF00342196
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DOI: https://doi.org/10.1007/BF00342196