Summary
A tyrosyl-tRNA synthetase mutant of Escherichia coli was isolated and the tyrS gene assigned a map position between man and pdxH at 36.0 min on the chromosome. The tyrS mutant grew badly on broth as did previously described tyrS mutants. This sensitivity to broth was suppressed by tyrR mutations. F-prime factors were found to complement the tyrS mutation.
Similar content being viewed by others
References
Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of E. coli K-12. Bacteriol. Rev. 40, 116–166 (1976)
Buonocore, V., Harris, M.H., Schlesinger, S.: Properties of tyrosyltransfer ribonucleic acid synthetase from two tyrS mutants of E. coli K-12. J. Biol. Chem. 247, 4843–4849 (1972)
Diderichsen, B., Fiil, N.P., Lavallé, R.: Genetics of the relB locus in E. coli. J. Bacteriol. 131, 30–33 (1977)
Ehresmann, B., Imbault, P., Weil, J.H.: Spectrophotometric determination of protein concentration in cell extracts containing tRNA's and rRNA's. Anal. Biochem. 54, 454–463 (1973)
Glansdorff, N.: Topography of cotransducible arginine mutations in E. coli K-12. Genetics 51, 167–179 (1965)
Hanson, R.L., Rose, C.: Genetic mapping of a mutation affecting pyridine nucleotide transhydrogenase in E. coli. J. Bacteriol. 138, 783–787 (1979)
Low, K.B.: E. coli K-12 F-prime factors, old and new. Bacteriol. Rev. 36, 587–607 (1972)
Miller, J.H.: Experiments in molecular genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1972
Novel, M., Novel, G.: Regulation of β-glucuronidase synthesis in E. coli K-12: Constitutive mutants specifically derepressed for uidA expression. J. Bacteriol. 127, 406–417 (1976)
Schlesinger, S., Nester, E.W.: Mutants of E. coli with an altered tyrosyl-transfer ribonucleic acid synthetase. J. Bacteriol. 100, 167–175 (1969)
Wallace, B.J., Pittard, J.: Regualator gene controlling enzymes concerned in tyrosine biosynthesis in E. coli. J. Bacteriol. 97, 1234–1241 (1969)
Yem, D.W., Wu, H.C.: Physiological characterization of an E. coli mutant altered in the structure of murein lipoprotein. J. Bacteriol. 133, 1419–1426 (1978)
Author information
Authors and Affiliations
Additional information
Communicated by A. Böck
Rights and permissions
About this article
Cite this article
Diderichsen, B., De Hauwer, G. Improved mapping of the tyrS locus in Escherichia coli . Molec. gen. Genet. 178, 647–650 (1980). https://doi.org/10.1007/BF00337873
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00337873