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DNA amplification and an unstable arginine gene in Streptomyces lividans 66

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Summary

Streptomyces lividans 66 produced spontaneous chloramphenicol-sensitive mutants (Cmls) at a frequency of about 1% of spores. The Cmls mutant strains were very unstable, giving Arg- mutants at frequencies of about 25% of spores. All the Arg- mutants had amplified a particular 5.75 kb DNA fragment into tandem repeats of 250–500 copies per chromosome.

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Authors and Affiliations

  1. Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, P.O. Box 88, M60 1QD, Manchester, UK

    Josef Altenbuchner & John Cullum

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  1. Josef Altenbuchner
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  2. John Cullum
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Communicated by H. Saedler

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Altenbuchner, J., Cullum, J. DNA amplification and an unstable arginine gene in Streptomyces lividans 66 . Mol Gen Genet 195, 134–138 (1984). https://doi.org/10.1007/BF00332735

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  • DOI: https://doi.org/10.1007/BF00332735

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