Summary
The time course of REV2 dependent recovery from prelethal UV damage and UV-induced locus-specific reversion of the his5-2 allele was determined in temperatureshift experiments by use of a thermoconditional allele of the rev2 gene (rad5-8, rev2 ts). In UV-irradiated, exponentially growing rev2 ts cells the REV2 dependent repair activity persists for up to 8 h at permissive temperature (23° C), while the REV2 dependent mutagenic process is mostly completed within 2 h. The REV2 dependent process in exponentially growing cells is highly impaired by inhibition of protein synthesis. However, a REV2 dependent repair activity independent of de novo synthesis is detectable, even in the presence of up to 200 μg/ml cycloheximide, a response not found in stationary phase cells. Thus, the REV2 dependent process seems to be partially constitutive in exponentially growing cells. Additionally, exponentially growing rev2 ts cells were considerably more UV-sensitive at restrictive temperature (36°C) than were stationary phase cells.
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Siede, W., Eckardt, F. & Brendel, M. Analysis of mutagenic DNA repair in a thermoconditional repair mutant of Saccharomyces cerevisiae . Mol Gen Genet 190, 413–416 (1983). https://doi.org/10.1007/BF00331069
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DOI: https://doi.org/10.1007/BF00331069