Skip to main content
SpringerLink
Log in

Major chromosomal length polymorphisms are evident after meiosis in the phytopathogenic fungus Leptosphaeria maculans

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Abstract

Chromosomal DNA of Australian field-isolates of the phytopathogenic ascomycete Leptosphaeria maculans was resolved by pulsed-field gel electrophoresis. All isolates examined had highly variable karyotypes. Ascospores (sexual spores) derived from single pseudothecia (sexual fruiting bodies) isolated from Brassica napus (oilseed rape) stubble were analyzed. In two tetrads four distinct karyotypes were observed, with only one chromosomal DNA band in common to all the members of each tetrad. Although isolates had highly variable karyotypes, two overall patterns were present. In one pattern there were at least 12 chromosomal DNA bands, the largest being greater than 2.2 Mb in size; in the other there were more than 15 chromosomal DNA bands, the largest being about 2.0 Mb. The chromosomal DNA preparations included mitochondrial DNA which migrated as a diffuse band between 0.10 and 0.15 Mb in size, and DNA molecules of 8 and 9 kb in size.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Braithewaite KS, Irwin JAG, Manners JM (1990) Mycol Res 94:1129–1137

    Google Scholar 

  • Bartnik E, Pieniazek NJ, Stepien PP (1979) Mol Gen Genet 171:75–78

    Google Scholar 

  • Cargeeg LA, Thurling N (1980) Euphytica 29:465–476

    Google Scholar 

  • Cooley RN, Caten CE (1991) Mol Gen Genet 228:17–23

    Google Scholar 

  • Corcoran LM, Thompson J, Walliker D, Kemp DJ (1988) Cell 53:807–813

    Google Scholar 

  • Goodwin PH, Annis SL (1991) Appl Environ Microbiol 57:2482–2486

    Google Scholar 

  • Gunderson K, Chu G (1991) Mol Cell Biol 11:3348–3354

    Google Scholar 

  • Hamer JE, Farrall L, Orbach MJ, Valent B, Chumley FG (1989) Proc Natl Acad Sci USA 86:9981–9985

    Google Scholar 

  • Hassan AK, Schultz C, Sacristan MD, Wöstemeyer J (1991) J Phytopathol 131:120–136

    Google Scholar 

  • Howlett BJ (1989) Exp Mycol 13:199–202

    Google Scholar 

  • Howlett BJ, Brownlee AG, Guest DI, Adcock GJ, McFadden GI (1992) Curr Genet 22:455–461

    Google Scholar 

  • Johnson RD, Lewis BG (1990) Physiol Mol Plant Pathol 37:417–424

    Google Scholar 

  • Jorgensen RA, Cuellar RE, Thompson WE, Kavanagh TA (1987) Plant Mol Biol 8:3–12

    Google Scholar 

  • Kistler HC, Miao VPW (1992) Annu Rev Phytopathol 30:131–152

    Google Scholar 

  • Koch E, Song K, Osborn TC, Williams PH (1991) Mol Plant-Microbe Interact 4:341–349

    Google Scholar 

  • Lee SB, Taylor JW (1990) In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications, Academic Press, San Diego, pp. 282–287

    Google Scholar 

  • McCluskey K, Russell B, Mills D (1990) Curr Genet 18:385–386

    Google Scholar 

  • McDonald BA, Martinez JP (1991) Curr Genet 19:265–271

    Google Scholar 

  • McGee DC (1977) Aust J Agric Res 28:53–62

    Google Scholar 

  • Maleszka R, Clark-Walker GD (1992) Curr Genet 21:283–288

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York

    Google Scholar 

  • Masel A, Braithwaite K, Irwin J, Manners J (1990) Curr Genet 18:81–86

    Google Scholar 

  • Masel A, Irwin J, Manners J (1992) Mol Gen Genet (in press)

  • Meinhardt F, Kempken F, Kamper J, Esser K (1990) Curr Genet 17:89–95

    Google Scholar 

  • Miao VPW, Matthews DE, VanEtten HD (1991a) Mol Gen Genet 226:214–223

    Google Scholar 

  • Miao VPW, Covert SF, VanEtten HD (1991b) Science 254:1773–1776

    Google Scholar 

  • Mills D, McClusky K (1990) Mol Plant-Microbe Interact 3:351–357

    Google Scholar 

  • Orbach MJ, Vollrath D, Davis RW, Yanofsky C (1988) Mol Cell Biol 8:1469–1473

    Google Scholar 

  • Pang ECK (1992) PhD thesis, University of Melbourne

  • Petrie GA, Lewis PA (1985) Can J Plant Pathol 7:253–255

    Google Scholar 

  • Romao J, Hamer JE (1992) Proc Natl Acad Sci USA 89:5316–5320

    Google Scholar 

  • Salisbury P, Ballinger D, Hannah M (1989) Abstract 7th Aust Res Assembly on Brassicas, Townsville

  • Saiki RK, Gelfand DH, Stoffel S, Scharf S, Higuchi R, Horn G, Mullis K, Erlich HA (1988) Science 239:487–491

    Google Scholar 

  • Skinner DS, Budde AD, Leong SA (1991) Molecular karyotype analysis of fungi. In Bennett JW, Lasure LL (eds) More gene manipulations of fungi. Academic Press, New York, pp 86–103

    Google Scholar 

  • Talbot NJ, Oliver RP, Coddington A (1991) Mol Gen Genet 229:267–272

    Google Scholar 

  • Taylor JL, Borgmann I, Séguin-Swartz G (1991) Curr Genet 19:273–277

    Google Scholar 

  • Tzeng T-H, Lyngholm LK, Ford CF, Bronson CR (1992) Genetics 130:81–96

    Google Scholar 

  • Valent B, Chumley FG (1991) Annu Rev Phytopathol 29:443–467

    Google Scholar 

  • Venn L (1979) Aust Plant Pathol 8:5–6

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3052, Victoria, Parkville, Australia

    Kim M. Plummer & Barbara J. Howlett

Authors
  1. Kim M. Plummer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Barbara J. Howlett
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by O. C. Yoder

Rights and permissions

Reprints and permissions

About this article

Cite this article

Plummer, K.M., Howlett, B.J. Major chromosomal length polymorphisms are evident after meiosis in the phytopathogenic fungus Leptosphaeria maculans . Curr Genet 24, 107–113 (1993). https://doi.org/10.1007/BF00324673

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00324673

Key words

Search

Navigation