Abstract
In the course of studying sir4-11 which is responsible for the non-mating phenotype of asd-homothallism of Saccharomyces cerevisiae, we detected chromosomal α-specific suppressors of it. By examining the mating-type cassette constitution of two strains and the spore-clones derived from a diploid culture formed by a mass mating between these strains, we obtained the following results; (1) the HMRa-bearing HindIII-BglII restriction fragment was dimorphic, as judged by size, within each tetrad, (2) while one form was common to all tetrads, the other varied among tetrads, (3) spore-clones with the α-specific suppressor of sir4-11 had the variant forms while those without had the common form, and (4) while the two parents had the common form, each independent diploid clone had the common form plus a variant form. From these results, we conclude that the mating of cells in certain combinations induces a change of DNA structure at or near HMRa in a mating-pair specific manner and that the change makes HMRa non-derepressible or non-functional when derepressed.
Similar content being viewed by others
References
Astell CR, Ahlstrom-Jonesson L, Smith L, Tatchell K, Nasmyth KA, Hall BD (1981) Cell 27:15–23
Hanahan D (1983) J Mol Biol 166:557–580
Hereford LK, Fahrner K, Woodford Jr J, Rosbach M, Kaback DB (1979) Cell 18:1261–1271
Klar AJS, Fogel S, MacLeod K (1979) Genetics 93:37–50
Nasmyth KA (1982) Cell 30:567–578
Nasmyth KA, Tatchell K (1980) Cell 19:753–764
Ono B, Ishino-Arao Y, Takasugi K, Taniguchi M, Fukuda M, Miyakawa I, Sando N (1990) Genetics 125:729–738
Ono B, Ishino-Arao Y, Taniguchi M (1993) Curr Genet 24:1–6
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sherman F, Fink GR, Hicks JB (1986) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sor F, Cheret G, Fabre F, Faye G, Fukuhara H (1992) Yeast 8:215–222
Southern EM (1975) J Mol Biol 98:503–517
Sundmeyer SB, Hansen LJ, Chalker DL (1990) Annu Rev Genet 24:491–518
Tanaka K, Oshima T, Araki H, Harashima S, Oshima Y (1984) Mol Cell Biol 4:203–211
Yamane A, Nakagami S, Miyoshi K (1988) Nucleic Acids Res Symp Ser 19:93–95
Author information
Authors and Affiliations
Additional information
Communicated by B.S. Cox
Rights and permissions
About this article
Cite this article
Ono, Bi., Takasugi, K., Ishino-Arao, Y. et al. Relationship between chromosomal α-specific suppressors of sir4-11 and polymorphism of the HMRa-bearing fragment. Curr Genet 24, 7–11 (1993). https://doi.org/10.1007/BF00324658
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00324658