Summary
In eight patients suffering from polycythaemia vera, the proliferation of erythroid colonies in methylcellulose with and without exogenous erythropoietin was studied. After three and five weeks, respectively, no colonies grew. Due to addition of erythropoietin more erythroid colonies proliferated which could be cultivated over nine weeks. The experiments suggest that the erythropoiesis in polycythaemia vera depends on erythropoietin.
This is a preview of subscription content, log in via an institution to check access.
Literatur
Adamson JW (1968) The erythropoietin-hematocrit relationship in normal and polycythemic man: implications of marrow regulation. Blood 32: 597
Adamson JW (1968) Erythropoietin and the polycythaemias. Ann NY Acad Sci 149: 560
Adamson JW, Fialkow PJ, Murphy S, Prchal JF, Steinman L (1976) Polycythemia vera: stem-cell and probable clonal origin of the disease. N Engl J Med 295: 913
Berlin NJ (1975) Diagnosis and classification of the polycythemias. Semin Hematol 12: 339
Clarke BJ, Housman D (1977) Characterization of an erythroid precursor cell of high proliferative capacity in normal human peripheral blood. Proc Natl Acad Sci USA 74: 1105
Guilbert LJ, Iscove NN (1976) Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoietic cell cultures. Nature 263: 594
Heilmann E, Essers U (1980) Proliferationsverhalten hämatopoetischer Vorstufen in Methylzellulose-Kulturen bei Polycythämia vera. Verh Dtsch Ges Inn Med 86:1087
Heilmann E, Holzknecht A, Fahrenkrug H (1981) Hinweise auf einen hämatopoetisch wirksamen Faktor im Serum von Patienten mit Polycythämia vera. Verh Dtsch Ges Inn Med (in press)
Hodgson GS, Bradley TR, Radley JM (1980) (Abstract) Evidence for an 》age structured《 haemopoietic stem cell compartment, of which CFU-S are only a subset. 18th Congr Int Soc Hematol, p 89
Iscove NN, Senn JS, Till JE, McCulloch EA (1971) Colony formation by normal and leukemic human marrow cells in culture, effect of conditioned medium from human leukocytes. Blood 37: 1
Kluge N, Knebel A, Beckmann H (1973) Polycythämia vera: Neoplastische Transformation von Knochenmarkszellen in vitro. Verh Dtsch Ges Inn Med 441: 79
Kubanek B, Bock E (1976) Erythropoetinausscheidung als differentialdiagnostisches Kriterium zur Abgrenzung von Polyglobulien und Polycythaemia vera. In: Stacher A, Höcker P (Hrsg) Erkrankungen der Myelopoese. Urban u. Schwarzenberg, München Wien Baltimore, p 480
Lutton JD, Levere RD (1979) Endogenous Erythroid Colony Formation by Peripheral Blood Mononuclear Cells from Patients with Myelofibrosis and Polycythaemia vera. Acta Haematol 62:94
Nissen C, Cornu P, Weber W, Speck B (1978) Differentialdiagnose der primären und sekundären Erythrozytosen mit Hilfe der In-Vitro-Kultur hämopoetischer Vorläuferzellen. Schweiz Med Wochenschr 108: 1581
Prchal JF, Adamson JW, Murphy S, Steinmann L, Fialkow (1976) Polycythaemia vera: the in vitro response of normal and abnormal stem cell lines to erythropoietin. Clin Res 24: 442 A
Prchal JF, Axelrad AA (1974) Bone marrow responses in polycythaemia vera. N Engl J Med 290: 1382
Ward HP, Vauirin R, Kursnick J (1974) Presence of a myeloproliferative factor in patients with polycythaemia vera and agnogenic myeloid metaplasia. Proc Soc Exp Biol Med 147: 305
Zanjani NN, Lutton JD, Hoffmann R, Wassermann LR (1977) Erythroid colony formation by polycythaemia vera bone marrow in vitro. J Clin Invest 59: 841
Author information
Authors and Affiliations
Additional information
Mit Unterstützung des Landesamtes für Forschung NW
Rights and permissions
About this article
Cite this article
Heilmann, E., Holzknecht, A., Fahrenkrug, H. et al. Langzeitkulturen bei Polycythaemia vera zum Nachweis von Erythropoetinabhängigkeit. Blut 43, 51–56 (1981). https://doi.org/10.1007/BF00319932
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00319932