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Histochemical demonstration of carbonic anhydrase activity in the alimentary canal

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Carbonic anhydrase (CAH) activity was histochemically demonstrated in various parts of the alimentary canal of rat and in the stomach of man using the method of Waldeyer and Häusler (1959). The most intense histochemical reaction was observed in the parietal cells of the rat stomach, and reactions of decreasing intensity in the epithelial cells of the colon, appendix, jejunoileum, duodenum and oesophagus in the order mentioned. An intense reaction was also observed in the parietal cells of the stomach of man and a weak activity in the pyloric glands. After electrophoresis on cellulose acetate film the CAH activity in human and rat stomach mucosa showed one band with the same migration rate as the fastest moving band of the erythrocyte CAH.

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Korhonen, L.K., Korhonen, E. & Hyyppä, M. Histochemical demonstration of carbonic anhydrase activity in the alimentary canal. Histochemie 6, 168–172 (1966). https://doi.org/10.1007/BF00308189

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  • DOI: https://doi.org/10.1007/BF00308189

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