Summary
Ultrastructural aspects and cytochemical localisation of Ca-uptake in the fragmented sarcoplasmic reticulum (FSR) have been studied with positive and various negative staining techniques.
Effect of fixation, staining and other preparative procedures for electron microscopy on the result of the cytochemical reaction have been investigated.
Calcium outflow from loaded vesicles occurs in various degree depending on the method used so that no quantitative estimation of Ca-uptake appears possible by ultrastructural studies alone.
Ultrastructure and proportion of calcium containing vesicles also vary considerably and no certain correlations can be made between their size and shape and localisation of Ca-uptake. Certain informations can be obtained only by comparison of results with different methods, taking into account also the limits of each method applied.
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Agostini, B., Hasselbach, W. Electron cytochemistry of calcium uptake in the fragmented sarcoplasmic reticulum. Histochemie 28, 55–67 (1971). https://doi.org/10.1007/BF00305632
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DOI: https://doi.org/10.1007/BF00305632