Summary
Prenatal and postnatal stages of the development of golden hamsters were studied histochemically and biochemically. It was shown that, beginning with the 12th gestational day, the fetal liver starts to store glycogen, and that this process reaches its maximum a birth. Glycogen phosphorylase and glucose-6-phosphatase (G6Pase)-activity increased drastically in the last two days before birth, glycogen phosphorylase preceding G6Pase. As a histochemical characteristic, an even distribution of glycogen, glycogen phosphorylase and G6Pase activity is found in the liver parenchyma at birth. During the first two postnatal weeks typical heterogeneous patterns of distribution developed: glycogen depletion could be demonstrated predominantly in zone 1 of the liver acinus, this being at the same time the area of highest glycogen phosphorylase and G6Pase-activity. The periportal zone 1 thus became characterized as the primary site of glycogenolysis (glycogen phosphorylase) and gluco(neo)genesis (G6Pase). “Metabolic Zonation” is interpreted as the chemomorphological equivalent of the regulatory function of the liver as a glucostat.
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References
Gomori, G.: In Histochemistry, theoretical and applied (A.G.E. Pearse, ed.) Vol. I. Boston: Little Brown and Company 1968
Graumann, W.: Zur Standardisierung des Schiffschen Reagens. Z. wiss. Mikr. 61, 225–226 (1953)
Greengard, O.: Enzymic differentiation in mammalian liver. Science 163, 891–895 (1969)
Guder, W., Schmidt, U.: Liver cell heterogeneity of pyruvate kinase and phosphoenolpyruvate carboxykinase (GTP) in the liver lobule of fed and starved rats. Hoppe Seylers's. Z. Physiol. Chem. 357, 1793–1800 (1976)
Jungermann, K., Katz, N., Teutsch, H.F., Sasse, D.: Possible metabolic zonation of liver parenchyma into glucogenic and glycolytic hepatocytes. In: Alcohol and aldehydes metabolizing systems (Thurman, R.G., Williamson, J.R., Drott, H., Chance, B., eds.) Vol. 3, pp. 65–76 New York: Academic Press 1977
Jungermann, K., Sasse, D.: Heterogeneity of liver parenchymal cells. Trends Biochem. Sci. 3, 198–202 (1978)
Katz, N., Teutsch, H.F., Jungermann, K., Sasse, D.: Perinatal development of the metabolic zonation of hamster liver parenchyma. FEBS Letters 69, 23–26 (1976)
Katz, N., Teutsch, H.F., Sasse, D., Jungermann, K.: Heterogeneous distribution of glucose-6-phosphatase in microdissected periportal and perivenous rat liver tissue. FEBS Letters 76, 226–230 (1977a)
Katz, N., Teutsch, H.F., Jungermann, K., Sasse, D.: Heterogeneous reciprocal localization of fructose-1,6-bisphosphatase and of glucokinase in microdissected periportal and perivenous rat liver tissue. FEBS Letters 83, 272–276 (1977b)
Keppler, D., Decker, K.: Glykogen. Bestimmung mit Amyloglucosidase. In: Methoden der enzymatischen Analyse (H.U. Bergmeier, ed.) 3. Aufl. p. 1171. Weinheim: Verlag Chemie 1974
Lindberg, L.A., Palkama, A.: Methodological observations on the histochemical demonstration of glycogen phosphorylase activity. Ann. med. exp. Fenn. 48/2, 67–76 (1970)
McManus, J.F.A.: Histological demonstration of mucin after periodic acid. Nature 158, 202 (1946)
Rappaport, A.M.: The microvasculatory acinar concept of normal and pathological hepatic structure. Beitr. Path. 157, 215–243 (1976)
Sasse, D.: Chemomorphologie der Glykogensynthese und des Glykogengehalts während der Histogenese der Leber. Histochemie 20, 159–170 (1969a)
Sasse, D.: Histochemische Untersuchungen zur funktionellen Gliederung des Leberparenchyms. Anat. Anz. (Suppl.) 125, 111–113 (1969b)
Sasse, D.: Dynamics of liver glycogen. The topochemistry of glycogen synthesis, glycogen content and glycogenolysis under the experimental conditions of glycogen accumulation and depletion. Histochemistry 45, 237–254 (1975)
Sasse, D., Katz, N., Jungermann, K.: Functional heterogeneity of rat liver parenchyma and of isolated hepatocytes. FEBS Letters 57, 83–88 (1975)
Takeuchi, T., Kuriaki, H.: Histochemical demonstration of phosphorylase in animal tissues. J. Histochem. Cytochem. 3, 153–160 (1955)
Teutsch, H.F.: Quantitative determination of G6Pase activity in histochemically defined zones of the liver acinus. Histochemistry 58, 281–288 (1978)
Wachstein, M., Meisel, E.: On the histochemical demonstration of glucose-6-phosphatase. J. Histochem. Cytochem. 4, 592 (1956)
Ward, M.C.: The early development and implantation of the golden hamster, Cricetus auratus, and the associated endometrial changes. Amer. J. Anat. 82, 231–276 (1948)
Wimhurst, J.M., Manchester, K.L.: A comparison of the effects of diabetes induced with either alloxan or streptozotocin and of starvation on the activities in rat liver of the key enzymes of gluconeogenesis. Biochem. J. 120, 95–103 (1970)
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Dedicated to Prof. Dr. A.M. Rappaport on the occasion of his 75th birthday, with sincere thanks for many years of fruitful discussion
Supported by a grant from the SFB 46 (“Molgrudent”)
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Sasse, D., Teutsch, H.F., Katz, N. et al. The development of functional heterogeneity in the liver parenchyma of the golden hamster. Anat Embryol 156, 153–163 (1979). https://doi.org/10.1007/BF00300011
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DOI: https://doi.org/10.1007/BF00300011