Summary
Four different human tumor cell lines of glial origin have been exposed to a human T lymphotropic retrovirus (HTLV-I). All these cell lines were positive for the glial marker glial fibrillary acidic protein (GFAP). The presence of virus RNA was demonstrated by in situ hybridization using an HTLV-I, SStI-SStI viral insert as probe. Virus expression has been monitored through an indirect immunofluorescence assay using a monoclonal antibody against virus core protein p19. All the four glioma cell lines tested became positive for p19 after 2 weeks of co-cultivation and showed a clear alteration of GFAP expression.
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Supported in part by the Ministero della Sanità, Projects AIDS and by MURST 40% Grant
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Macchi, B., Caronti, B., Pezzella, M. et al. Effect of human T lymphotropic retrovirus-I exposure on cultured human glioma cell lines. Acta Neuropathol 81, 670–674 (1991). https://doi.org/10.1007/BF00296378
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DOI: https://doi.org/10.1007/BF00296378