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In vitro synthesis and repression of argininosuccinase in Escherichia coli K12; partial purification of the arginine repressor

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Summary

Φ80dargECBH DNA has been used to direct cell-free synthesis of argininosuccinase, the argH gene product in Escherichia coli K12. In vitro enzyme synthesis is sensitive to repression by partially purified preparations from an argR + strain but not by corresponding preparations from an argR - strain. Using DNA-cellulose chromatography, approximately seventyfold purification of repressor has been obtained. The partially purified preparation represses argininosuccinase synthesis but has no effect on β-galactosidase synthesis.

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Authors and Affiliations

  1. Department of Microbiology, New York University School of Medicine, 10016, New York, New York, U.S.A.

    Norman E. Kelker & Werner K. Maas

  2. Department of Biological Sciences, Columbia University, 10027, New York, New York, U.S.A.

    Huey-Lang Yang & Geoffrey Zubay

Authors
  1. Norman E. Kelker
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  2. Werner K. Maas
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  3. Huey-Lang Yang
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  4. Geoffrey Zubay
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Communicated by W. Gilbert

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Kelker, N.E., Maas, W.K., Yang, HL. et al. In vitro synthesis and repression of argininosuccinase in Escherichia coli K12; partial purification of the arginine repressor. Molec. Gen. Genet. 144, 17–20 (1976). https://doi.org/10.1007/BF00277298

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  • DOI: https://doi.org/10.1007/BF00277298

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