Summary
Insulin receptors from H-2k mouse liver membranes were specifically labelled using a photoreactive insulin analogue coupled to the α-subunit (Mr 130,000) of the receptor by ultraviolet-irradiation. Up to 25% of the labelled insulin receptors recovered after membrane solubilization and precipitable by anti-insulin receptor antibodies could be immunoprecipitated by various monoclonal antibodies reacting with H-2K+ antigens. None of these monoclonal antibodies inhibited insulin binding to its receptor. These results show that insulin receptors and H-2Kk antigens interact in liver plasma membranes to form non-covalent complexes. This association between insulin receptors and major histocompatibility complex antigens could be involved in the pathogenesis of certain forms of insulin resistance.
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Brossette, N., Van Obberghen, E. & Fehlmann, M. Interaction between insulin receptors and major histocompatibility complex antigens in mouse liver membranes. Diabetologia 27 (Suppl 1), 74–76 (1984). https://doi.org/10.1007/BF00275651
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DOI: https://doi.org/10.1007/BF00275651