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In vitro propagation through axillary bud multiplication in different mulberry genotypes

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Abstract

Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25–0.5 mg/l), and in M. alba and M. rotondifolia GA3 (0.5–1.0 mg/l)were found to induce sprouting. Two genotypes of M. bombycis, namely Schimanochi and Mizusawa, developed healthy shoots on the incorporation of 2,4-D (0.5–1.0 mg/l) and BAP (0.5–2.0 mg/l), respectively. IBA (0.5 mg/l), along with cytokinin/auxin/gibberellin, had no effect on bud growth but helped root induction. Shoots developed from the axillary buds were further multiplied as nodal explants. MS basal medium supplemented with 0.5 mg/l IBA and LS vitamins was found best to produce healthy plantlets in all the genotypes. An average 89% survival was observed on transferring the plantlets to soil.

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Abbreviations

MS:

Murashige and Skoog (1962)

LS:

Linsmaier and Skoog (1965)

IBA:

3-indole-butyric acid

GA3 :

Gibberellic acid

BAP:

6-Benzylaminopurine

Kn:

Kinetin

2,4-D:

2,4-Dichlorophenoxyacetic acid

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Authors and Affiliations

  1. Tissue Culture Laboratory, Mulberry Breeding and Genetics Section, Central Sericultural Research and Training Institute, 570008, Mysore, India

    A. K. Jain, S. B. Dandin & K. Sengupta

Authors
  1. A. K. Jain
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  2. S. B. Dandin
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  3. K. Sengupta
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Communicated by I. K. Vasil

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Jain, A.K., Dandin, S.B. & Sengupta, K. In vitro propagation through axillary bud multiplication in different mulberry genotypes. Plant Cell Reports 8, 737–740 (1990). https://doi.org/10.1007/BF00272107

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  • DOI: https://doi.org/10.1007/BF00272107

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