Abstract
We describe a simple and reliable technique for labeling Pseudomonas aeruginosa with a readily available commercial preparation of indium-111 (111In) oxine. Labeling of a heavy bacterial suspension with 500 μCi of commercially prepared 111In-oxine resulted in a yield of 0.0004 μCi of cell-associated 111In per 106 colony-forming units (CFU). The label was 88% bacterially associated and did not effect viability of the organism. Radiolabeling a gram-negative organism with 111In-oxine provides a nontoxic, stable gamma-emitting bacterial tracer.
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Bettin, K., Allen, M.O., Gerding, D.N. et al. In-111 Pseudomonas aeruginosa: A simple method of labeling live bacteria with a gamma-emitting radioisotope. Eur J Nucl Med 12, 277–279 (1986). https://doi.org/10.1007/BF00251987
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DOI: https://doi.org/10.1007/BF00251987