Summary
We report the regeneration of protoplasts isolated from two embryogenic cell lines of Gossypium hirsutum L. cv. Coker 312 initiated from hypocotylderived callus. Protoplasts plated on cellulose nitrate filters and placed over feeder layers formed embryogenic callus from which plants were regenerated. Plating efficiency up to 12.8% depended upon the cell line. Addition of phytohormones to the protoplast medium had no stimulating effect on plating efficiency. The influence of feeder cells and conditioned medium on plating efficiency was significantly different for the two cell lines.
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Abbreviations
- ACM:
-
autoclaved conditioned medium
- AFC:
-
autoclaved feeder cells
- BM:
-
basic medium
- BM+:
-
basic medium with phytohormones
- CM:
-
non-autoclaved conditioned medium
- FC:
-
non-autoclaved feeder cells
- FDA:
-
fluorescein diacetate
- MM:
-
maturation medium
- NAA:
-
1-naphtaleneacetic acid
- PCM:
-
protoplast culture medium
- PCM+:
-
protoplast culture medium with phytohormones
- SC:
-
settled cells
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- 6-BAP:
-
6-benzylamino purine
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Peeters, MC., Willems, K. & Swennen, R. Protoplast-to-plant regeneration in cotton (Gossypium hirsutum L. cv. Coker 312) using feeder layers. Plant Cell Reports 13, 208–211 (1994). https://doi.org/10.1007/BF00239894
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DOI: https://doi.org/10.1007/BF00239894