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Ultrastructural localization of carbonic anhydrase in tissues involved in shell formation and ionic regulation in the pond snail Lymnaea stagnalis

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Summary

The ultrastructural localization of carbonic anhydrase (CA) was investigated with the cobalt-bicarbonate method in three epithelia of the pond snail Lymnaea stagnalis. In the epidermis a selective population of “positive cells” was observed. In these cells, CA is confined to the apical and to small parts of the lateral plasma membrane. In cells of the outer mantle epithelium, CA is localized in the lateral and basal parts of the plasma membrane. In cells of the ureter, CA was found apically as well as basally. The localization of CA is discussed in relation to the different functions of the epidermis (electrolyte uptake), mantle (HCO −3 secretion, calcification) and ureter (electrolyte uptake, acid-base regulation).

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Authors and Affiliations

  1. Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands

    H. H. Boer & J. Witteveen

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  1. H. H. Boer
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  2. J. Witteveen
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The authors wish to thank Prof. J. Joosse, Dr. A.A. Dogterom and Dr. N.D. de With for reading the manuscript, Mrs. Dagmara Reichelt for technical assistance and Mr. G.W.H. van den Berg for drawing Fig. 5

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Boer, H.H., Witteveen, J. Ultrastructural localization of carbonic anhydrase in tissues involved in shell formation and ionic regulation in the pond snail Lymnaea stagnalis . Cell Tissue Res. 209, 383–390 (1980). https://doi.org/10.1007/BF00234753

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