Abstract
A rapidly growing embryogenic suspension culture cell line of creeping bentgrass cv Penncross (Agrostis palustris Huds.) was established from callus derived from the culture of mature seeds. High concentrations of 2,4-D were required for the induction of callus (3 mg/1) as well as for the maintenance of the cell Une (2 mg/1) on modified B5 medium of Gamborg. Protoplasts isolated from the suspension cultures were successfully cultured in Murashige and Skoog's basal medium supplemented with only 0.1 mg/1 2,4-D. Although protoplast plating efficiency was rather low (0.36%), 30% of the protocalli formed normal green plants that were successfully established in soil.
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Abbreviations
- BA:
-
6, benzylaminopurine
- 2,4-D:
-
2,4 dichlorophenoxyacetic acid
- MES:
-
2, (N-morpholino)-ethane sulfonic acid
- MS:
-
Murashige and Skoog medium (1962)
- B5:
-
Gamborg medium (1968)
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Communicated by I. K. Vasil
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Terakawa, T., Sato, T. & Koike, M. Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris Huds.). Plant Cell Reports 11, 457–461 (1992). https://doi.org/10.1007/BF00232690
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DOI: https://doi.org/10.1007/BF00232690