Summary
The effects of high-concentration, 2-h liquid pulses of N6-benzylaminopurine (BA) and thidiazuron (TD) on adventitious bud and shoot formation were tested in cotyledons of Douglas-fir (Pseudotsuga menziesii). Seedling age proved important; on average, cotyledons from the youngest seedlings formed 10-fold more buds than cotyledons from the oldest seedlings. Optimal cytokinin concentrations for the youngest cotyledons were 400 and 800 μM BA, and 100 and 200 μM TD. Shoots developed best from buds induced with 300, 400, and 800 μM BA. Four gelling agents were tested; BRL agarose yielded more than three times the number of buds, and Gelrite nearly twice the number of buds, as either Sigma agar or Difco Bacto-Agar. One of the best treatments (400 μM BA, agarose) yielded more cotyledons with buds, and more buds per cotyledon, than when cytokinins were incorporated into the growth medium.
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Abbreviations
- BA:
-
N6-benzylaminopurine
- TD:
-
thidiazuron
- SH:
-
Schenk and Hildebrandt (1972)
- NAA:
-
1-naphthaleneacetic acid
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Communicated by F. Constabel
Paper 2691 of the Forest Research Laboratory, Oregon State University, Corvallis. Mention of trade names or commercial products does not constitute endorsement or recommendation for use by the authors or Oregon State University.
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Goldfarb, B., Howe, G.T., Bailey, L.M. et al. A liquid cytokinin pulse induces adventitious shoot formation from Douglas-fir cotyledons. Plant Cell Reports 10, 156–160 (1991). https://doi.org/10.1007/BF00232049
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DOI: https://doi.org/10.1007/BF00232049