Summary
High pressure exposure produces neurological changes which manifest as tremors, EEG changes and convulsions. Since previous studies have implicated the involvement of the monoaminergic system in these symptoms, it was of interest to study monoamine release at high pressure. Synaptosomes isolated from guinea pig brain were used to follow monoamine efflux at 68 ATA. The major observation was a decrease in the initial calcium dependent release of all three monoamines in response to K+ induced depolarization. This response is similar to that previously observed for GABA, glycine and glutamate. This generalized pressure induced depression of initial transmitter release suggests a mechanism common to the release process for both excitatory and inhibitory neurotransmission.
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References
Augustine GJ, Charlton MP, Smith SJ (1987) Calcium action in synaptic transmitter release. Ann Rev Neurosci 10:633–693
Barnes DM (1988) New data intensify the agony over ecstacy. Science 239:864–866
Bennett PB, McLeod J (1984) Probing the limits of human deep diving. Philos Trans R Soc Land B304:105–117
Blaustein MP, Kendrick NC, Fried RC, Ratzlaft RW (1977) Calcium metabolism at the mammalian presynaptic nerve terminal: lessons from the synaptosome. In: Cotman WM, Ferrendelli JA (eds) Approaches to the cell biology of neurons. Society for Neuroscience, Bethesda, pp 149–159
Bowser-Riley F, Dobbie JA, Paton WDM, Smith EB (1982) A possible role for monoaminergic inhibition in the high pressure neurological syndrome. Undersea Biomed Res 9:32–33
Bowser-Riley F (1984) Mechanistic studies on the high pressue neurological syndrome. Philos Trans R Soc Lond B 304:31–41
Brauer RW, Beaver RW, Sheehan ME (1978) Role of monoamine neurotransmitters in the compression rate dependence of HPNS convulsions. In: Shilling CW, Bennett MW (eds) Proceedings of the 6th symposium on underwater physiology. Federation of American Societies for Experimental Biology, Bethesda, pp 49–60
Cotman CW, Haycock JW, White WF (1976) Stimulus-secretion coupling processes in brain: analysis of noradrenaline and gamma-aminobutyric acid release. J Physiol 254:475–505
Debelleroche JS, Bradford HF (1973) The synaptosome: an isolated, working neuronal compartment. Prog Neurobiol 1:277–298
DeLorenzo RJ (1980) Role of calmodulin in neurotransmitter release and synaptic function. Ann NY Acad Sci 356:92–109
Gilman SC, Kumaroo KK, Hallenbeck JM (1986a) Effects of pressure on uptake and release of calcium by brain synpatosomes. J Appl Physiol 60:1446–1450
Gilman SC, Colton JS, Hallenbeck JM (1986b) Effect of pressure on [3H]GABA release by synaptosomes isolated from the cerebral cortex. J Appl Physiol 61(6):2067–2073
Gilman SC, Colton JS, Dutka AJ (1987) Effect of pressure on the release of radioactive glycine and GABA from spinal cord synaptosomes. J Neurochem 49:1571–1578
Hallenbeck JM (1981) A view of some fundamentals of the high pressure nervous syndrome. In: Bachrach AJ, Matzen MM (eds) Undersea physiology. Undersea Medical Society, Bethesda, pp 305–315
Heikkila RE (1988) 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (MPTP): a dopaminergic neurotoxin. Neurotransmissions 4:1–4
Heinemann SH, Conti F, Stuhmer W, Neher E (1987) Effects of hydrostatic pressure on membrane processes: sodium channels, calcium channels, and exocytosis. J Gen Physiol 90:765–778
Hunter WL, Bennett PB (1974) The causes, mechanisms, and prevention of the high pressure nervous syndrome. Undersea Biomed Res 1:1–28
Huttner WB, Schiebler W, Greengard P, DeCamilli P (1983) Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation. J Cell Biol 96:1374–1388
Koblin DD, Little HJ, Green AR, Daniels S, Smith EB, Paton WDM (1980) Brain monoamines and the high pressure neurological syndrome. Neuropharmacology 19:1031–1038
Langston JW, Ballard P, Tetrud JW, Irwin I (1983) Chronic parkinsonism in humans due to a product of meperidine synthesis. Science 219:979–980
Pollard HB, Rojas E, Burns AL (1987) Synexin and chromaffin granule membrane fusion: a novel “hydrophobic bridge” hypothesis for the driving and directing of the fusion process. Ann NY Acad Sci 493:524–541
Ricaurte G, Bryan G, Strauss L, Seiden L, Schuster C (1985) Hallucinogenic amphetamine selectively destroys serotonin nerve terminals. Science 229:986–988
Rutledge CO (1987) Effect of metabolic inhibitors and ouabain on amphetamine- and potassium-induced release of biogenic amines from isolated brain. Biochem Pharmacol 27:511–516
Steele TT, Nichols DE, Yim, GKW (1987) Sterochemical effects of 3,4-methylenedioxy-methamphetamine (MDMA) and related amphetamine derivatives on inhibition of uptake of [3H]monoamines into synaptosomes from different regions of rat brain. Biochem Pharmacol 36:2297–2303
Vaernes R, Hammerborg D, Ellertsen B, Peterson R, Tonjum S (1983) Central nervous system reactions during heliox and trimix dives to 51 ATA. Undersea Biomed Res 10:169–192
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Gilman, S.C., Colton, J.S. & Dutka, A.J. Alterations in brain monoamine neurotransmitter release at high pressure. Exp Brain Res 78, 179–184 (1989). https://doi.org/10.1007/BF00230697
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DOI: https://doi.org/10.1007/BF00230697