Summary
Anther culture, when applied to hexaploid triticale×wheat hybrids, offers the opportunity to re-assort wheat D genome and rye R genome chromosomes into homozygous doubled haploid lines in a single generation. The characterisation of such lines is the first step in their utilisation in wheat improvement. Two lines, M24 and M25 from the cross of ‘Beagle’בKedong 58’, and one line, M27, from the cross ‘Beagle’בJinghua No. 1’ have been characterised using different methods including conventional cytology and chromosome banding, and by using marker systems for storage protein composition (glutenins and gliadins), isozymes (α-amylase, aminopeptidase, glutamate oxaloacetic transaminase (GOT)) and RFLP markers. The results from all approaches were consistent in proving that M24 is a whole chromosome 6R/6D substitution line, while M25 and M27 are whole chromosome 1R/1D substitution lines. The relative advantages and disadvantages of each method of identification are also discussed.
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Communicated by G. Wenzel
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Tao, Y.Z., Snape, J.W. & Hu, H. The cytological and genetic characterisation of doubled haploid lines derived from triticale×wheat hybrids. Theoret. Appl. Genetics 81, 369–375 (1991). https://doi.org/10.1007/BF00228678
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DOI: https://doi.org/10.1007/BF00228678