Summary
Twenty-one celery (Apium graveolens L. var. dulce) cultivars, one celeriac (var. rapaceum) and one annual smallage (var. secalinum) cultivar were screened for polymorphic RAPD (Random Amplified Polymorphic DNA) markers with 28 arbitrary 10-mer primers. Among a total of 309 bands observed, 29 (9.3%) were polymorphic in the 23 cultivars screened, but only 19 (6.1%) markers were polymorphic within the 21 type dulce cultivars. These markers were sufficient to distinguish each of the cultivars used. The average marker difference was 6.4 between two celery cultivars, 16.7 between celery and annual smallage, 14.7 between celery and celeriac, and 12.0 between annual smallage and celeriac. The celery cultivars surveyed were classified into three groups based on the marker differences. The relationship among the dulce-type cultivars concluded from this research is basically consistent with the known lineage of the cultivars and the previous study using stem protein and isozyme markers. RAPD technology provides a new alternative for cultivar identification and classification in celery.
Similar content being viewed by others
References
Beckmann JS, Soller M (1983) Restriction fragment length polymorphism in genetic improvement: methodologies, mapping and cost. Theor Appl Genet 67:33–43
Guzman VL, Burdine HW, Harris ED, Orsenigo JR, Showalter RK, Thayer PL, Winchester JA, Wolf EA, Berger RD, Genung WG, Zitier TZ (1973) Celery production on organic soils of South Florida. Bull 757, Florida Agricultural Experiment Station
Heustis G, MacGrath M, Quiros CF (1992) Develoment of gentic markers in celery based on restriction fragment length polymorphisms. Theor Appl Genet (in press)
Hu J, Quiros CF (1991 a) Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep 10:505–511
Hu J, Quiros CF (1991 b) Molecular and cytological evidence of deletions in alien chromosomes for two monosomic addition lines of Brassica campestris-oleracea. Theor Appl Genet 81:221–226
Mitchelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Prot Natl Acad Sci 88:9828–9832
Orton TJ, Hulbert SH, Durgan ME, Quiros CF (1984) UC1, Fusarium Yellow-resistant celery breeding line. Hortiscience 19:594
Quiros CF (1992) Genetic improvement in celery. In: Kalloo Bergh BO (eds) Genetic improvement of vegetable crops. Pergamon Press, Oxford, UK (in press)
Quiros CF, McGrath M, Stiles J (1987 a) Use of stem proteins and isozymes for the identification of celery cultivars. Plant Cell Rep 6:114–117
Quiros CF, Douches D, D'Antonio V (1987 b) Inheritence of annual habit in celery: cosegregation with isozyme and anthocyanin markers. Theor Appl Genet 74:203–208
Tanksley SD, Orton TJ (1983) Isozymes in plant genetics and breeding. Elsevier, Amsterdam
Toth KF, Lacy ML (1992) Field Evaluation of celery germ plasm for resistant to fusarium oxysporum F. Sp. Apii Race 2. Res Rep 523, Agricultural Experiment Station, East Lansing, Michigan State University
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218
Williams JGK, Kubelik AE, Levak KJ, Rafalski JA, Tingey SC (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Wrigley CW, Batey IL, Skerritt JH (1987) Complementing traditional methods of identifying cereal varieties with novel procedures. Seed Sci Technol 15:679–688
Author information
Authors and Affiliations
Additional information
Communicated by H.F. Linskens
Rights and permissions
About this article
Cite this article
Yang, X., Quiros, C. Identification and classification of celery cultivars with RAPD markers. Theoret. Appl. Genetics 86, 205–212 (1993). https://doi.org/10.1007/BF00222080
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222080