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Autologous responses to human leukaemic cells in mixed leucocyte culture

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Summary

Cryopreserved leukaemic blasts and remission non-T cells from 22 patients with acute leukaemia (15 lymphocytic, 7 non-lymphocytic) were tested as stimulators of autologous remission T cells and normal allogeneic T cells in primary and secondary MLC. In most cases the autologous response elicited by leukaemic cells was less than or equal to that elicited by remission non-T cells. However, T cells from 2 patients in long-standing first remission from ANLL displayed greater proliferation in response to leukaemic blasts than to remission non-T cells in both primary and secondary MLC. The results are suggestive of sensitization of these 2 patients to leukaemia-specific antigens, but other possible explanations are discussed.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, The University of Adelaide, GPO Box 498, 5001, Adelaide, S. A., Australia

    Leonie K. Ashman & Denise E. O'Keefe

  2. Division of Haematology, Institute of Medical and Veterinary Sciences, Adelaide, South Australia

    Christopher A. Juttner

  3. Oncology Unit, Adelaide Children's Hospital, Adelaide, South Australia

    R. G. Toogood & Michael S. Rice

Authors
  1. Leonie K. Ashman
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  2. Denise E. O'Keefe
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  3. Christopher A. Juttner
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  4. R. G. Toogood
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  5. Michael S. Rice
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Additional information

Abbreviations used: MLC, mixed leucocyte culture; ANLL, acute non-lymphocytic leukaemia; ALL, acute lymphoblastic leukaemia; AMLR, autologous mixed lymphocyte reaction; NK cells, natural killer cells; MNC, mononuclear cells

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Ashman, L.K., O'Keefe, D.E., Juttner, C.A. et al. Autologous responses to human leukaemic cells in mixed leucocyte culture. Cancer Immunol Immunother 22, 95–99 (1986). https://doi.org/10.1007/BF00199121

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  • DOI: https://doi.org/10.1007/BF00199121

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