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Antibodies to CD45 and other cell membrane antigens in systemic lupus erythematosus

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Conclusions

The multivalency of cold-reactive IgM anti-lymphocyte autoantibodies, together with the local density of reactive antigens on the cell surface, may confer a capacity for a variety of immunoregulatory and non-specific physiological roles in the immune system and in SLE and other autoimmune diseases. Targets of interest in this regard include CD45, β 2 microglobulin, and surface immunoglobulin. IgG anti-lymphocyte autoantibodies, while more difficult to study, also exhibit interesting specificities. However, whether any of the mechanisms by which anti-lymphocyte autoantibodies could alter cellular function actually obtain in vivo remains speculative. Essentially all of the data in this regard derive from experiments in which anti-lymphocyte autoantibody-containing SLE serum or plasma, or purified Ig fractions thereof, is combined with peripheral blood mononuclear cells in short-term culture in vitro. Thus, it is possible that anti-lymphocyte autoantibodies in SLE, rather than contributing to pathogenesis, reflect a physiological attempt by the immune system to restore homeostasis in the face of aggressive autoimmune stimulation.

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References

  1. Anderson CL, Stillman WS (1980) Raji assay for immune complexes. Evidence for detection of Raji-directed immunoglobulin G antibody in sera from patients with systemic lupus erythematosus. J Clin Invest 66:353

    Google Scholar 

  2. Arend WP, Emmerich TE, Sturge JC, Starkebaum GA (1977) Monocyte-reactive antibodies in patients with systemic lupus erythematosus. Arthritis Rheum 20:1049

    Google Scholar 

  3. Bell EB (1992) Function of CD4 T cell subsets in vivo: expression of CD45R isoforms. Semin Immunol 4:43

    Google Scholar 

  4. Beverley PC (1987) Human T cell subsets. Immunol Lett 14:26344

    Google Scholar 

  5. Beverley PC (1992) Functional analysis of human T cell subsets defined by CD45 isoform expression. Semin Immunol 4:35

    Google Scholar 

  6. Bluestein HG, Zvaifler NJ (1976) Brain-reactive lymphocytotoxic antibodies in the serum of patients with systemic lupus erythematosus. J Clin Invest 57:509

    Google Scholar 

  7. Butler WT, Sharp JT, Rossen RD (1972) Relationship of the clinical course of systemic lupus erythematosus to the presence of circulating lymphocytotoxic antibodies. Arthritis Rheum 15:231

    Google Scholar 

  8. Chien Y-H, Davis MM (1993) How alpha beta T-cell receptors “see” peptide/MHC complexes. Immunol Today 14:597

    Google Scholar 

  9. Evavold BD, Sloan-Lancaster J, Allen PM (1993) Tickling the TCR: selective T-cell functions stimulated by altered peptide ligands. Immunol Today 14:602

    Google Scholar 

  10. Forsyth KD, Chua K-Y, Talbot V, Thomas WR (1993) Expression of the leukocyte common antigen CD45 by endothelium. J Immunol 150:3471

    Google Scholar 

  11. Glinski W, Gershwin ME, Steinberg AD (1976) Fractionation of cells on a discontinuous Ficoll gradient. Study of subpopulations of human T cells using anti-T cell antibodies from patients with systemic lupus erythematosus. J Clin Invest 57:604

    Google Scholar 

  12. Jacob L, Lety M-A, Louvard D, Bach J-F (1985) Binding of a monoclonal anti-DNA autoantibody to identical protein(s) present at the surface of several human cell types involved in lupus pathogenesis. J Clin Invest 75:315

    Google Scholar 

  13. Kagami M, Koike T, Takabayashi K, Tomioka H, Yoshida S (1987) Antibody to activated lymphocytes in patients with systemic lupus erythematosus. J Rheumatol 14:907

    Google Scholar 

  14. Koretzky GA (1993) Role of the CD45 tyrosine phosphatase in signal transduction in the immune system. FASEB J 7:420

    Google Scholar 

  15. Kumagai S, Steinberg AD, Green I (1981) Antibodies to T cells in patients with systemic lupus erythematosus can induce antibody-dependent cell-mediated cytotoxicity against human T cells. J Clin Invest 67:605

    Google Scholar 

  16. Lafer EM, Rauch J, Andrzejewski C Jr, Mudd D, Furie B, Schwartz RS, Stollar BD (1981) Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids. J Exp Med 153:897

    Google Scholar 

  17. Lies RB, Messner RP, Williams RC Jr (1973) Relative T-cell specificity of lymphocytotoxins from patients with systemic lupus erythematosus. Arthritis Rheum 16:369

    Google Scholar 

  18. Litvin DA, Cohen PL, Winfield JB (1983) Characterization of warm-reactive IgG anti-lymphocyte antibodies in systemic lupus erythematosus. Relative specificity for mitogen-activated T cells and their soluble products. J Immunol 130:181

    Google Scholar 

  19. Maher GM, Hartman KR (1993) Detection of antineutrophil autoantibodies by flow cytometry: use of unfixed neutrophils as antigenic targets. J Clin Lab Anal 7:334

    Google Scholar 

  20. Marchalonis JJ, Kaymaz H, Dedeoglu F, Schluter SF, Yocum DE, Edmundson AB (1992) Human autoantibodies reactive with synthetic autoantigens from T-cell receptor beta chain. Proc Natl Acad Sci USA 89:3325

    Google Scholar 

  21. Marchalonis JJ, Kaymaz H, Schluter SF, Yocum DE (1993) Human autoantibodies to a synthetic putative T cell receptor β-chain regulatory idiotype: expression in autoimmunity and aging. Exp Clin Immunogenet 10:1

    Google Scholar 

  22. Marchalonis JJ, Schluter SF, Wilson L, Yocum DE, Boyer JT, Kay MMB (1993) Natural human antibodies to synthetic peptide autoantigens: correlations with age and autoimmune disease. Gerontology 39:65

    Google Scholar 

  23. Messner RP, DeHoratius RJ, Ferrone S (1980) Lymphocytotoxic antibodies in systemic lupus erythematosus patients and their relatives. Reactivity with the HLA antigenic molecular complex. Arthritis Rheum 23:265

    Google Scholar 

  24. Mimura T, Fernsten P, Jarjour W, Winfield JB (1990) Autoantibodies specific for different isoforms of CD45 in systemic lupus erythematosus. J Exp Med 172:653

    Google Scholar 

  25. Mittal KK, Rossen RD, Sharp JT, Lidsky MD, Butler WT (1970) Lymphocyte cytotoxic antibodies in systemic lupus erythematosus. Nature 225:1255

    Google Scholar 

  26. Morimoto C, Reinherz EL, Abe T (1980) Characteristics of anti-T cell antibodies in systemic lupus erythematosus: evidence for selective reactivity with normal suppressor cells defined by monoclonal antibodies. Clin Immunol Immunopathol 16:474

    Google Scholar 

  27. Morimoto C, Letvin NL, Distaso JA, Aldrich WR, Schlossman SF (1985) The isolation and characterization of the human suppressor inducer T cell subset. J Immunol 134:1508

    Google Scholar 

  28. Morimoto C, Steinberg AD, Letvin NL, Hagan M, Takeuchi T, Daley J, Levine H, Schlossman SF (1987) A defect of immunoregulatory T cell subsets in systemic lupus erythematosus patients demonstrated with anti-2H4 antibody. J Clin Invest 79:762

    Google Scholar 

  29. Noguchi M, Iwamori M, Hirano T, Kobayashi S, Hashimoto H, Hirose S, Nagai Y (1992) Autoantibodies to T and B cell lines detected in serum samples from patients with systemic lupus erythematosus with lymphopenia and hypocomplementaemia. Ann Rheum Dis 51:713

    Google Scholar 

  30. Okudaira K, Searles RP, Ceuppens JL, Goodwin JS, Williams RC Jr (1982) Anti-Ia reactivity in sera from patients with systemic lupus erythematosus. J Clin Invest 69:17

    Google Scholar 

  31. Ooi BS, Ooi YM, Pesce AJ, Pollak V (1977) Antibodies to β 2 microglobulin in the sera of patients with systemic lupus erythematosus. Immunology 33:535

    Google Scholar 

  32. Ozturk GE, Terasaki PI (1979) Non-HLA lymphocyte cytotoxins in various diseases. Tissue Antigens 14:52

    Google Scholar 

  33. Peake PW, Greenstein JD, Timmermans V, Gavrilovic L, Charlesworth JA (1988) Lymphocytotoxic antibodies in systemic lupus erythematosus: studies of their temperature dependence, binding characteristics, and specificity in vitro. Ann Rheum Dis 47:725

    Google Scholar 

  34. Pingel JT, Thomas ML (1989) Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation. Cell 58:1055

    Google Scholar 

  35. Pischel KD, Bluestein HG, Woods VL Jr (1984) Molecular characterization of lymphocyte cell surface proteins reactive with systemic lupus erythematosus (SLE) autoantibodies. Arthritis Rheum 27:83

    Google Scholar 

  36. Poggi A, Pardi R, Pella N, Morelli L, Sivori S, Vitale M, Revello V, Moretta A, Moretta L (1993) CD45-mediated regulation of LFA1 function in human natural killer cells. Anti-CD45 monoclonal antibodies inhibit the calcium mobilization induced via LFA1 molecules. Eur J Immunol 23:2454

    Google Scholar 

  37. Pope RM, Keightley R, McDuffy S (1982) Circulating autoantibodies to IgD in rheumatic diseases. J Immunol 128:1860

    Google Scholar 

  38. Ramirez F, Williams RC, Sibbitt WL, Searles RP (1986) Immunogloblin from systemic lupus erythematosus serum induces interferon release by normal mononuclear cells. Arthritis Rheum 29:326

    Google Scholar 

  39. Rekvig OP (1989) Intrinsic cell membrane antigen recognized by antichromatin autoantibodies; The membrane antigens do not derive from the nucleus. Scand J Immunol 29:7

    Google Scholar 

  40. Rekvig OP, Hannestad K (1977) Certain polyclonal anti-nuclear antibodies cross-react with the surface membrane of human lymphocytes and granulocytes. Scand J Immunol 6:1041

    Google Scholar 

  41. Rekvig OP, Hannestad K (1979) The specificity of human autoantibodies that react with both cell nuclei and plasma membranes: the nuclear antigen is present on core mononucleosomes. J Immunol 123:2673

    Google Scholar 

  42. Revillard JP, Vincent C, Rivera S (1979) Anti-β 2 microglobulin lymphocytotoxic autoantibodies in systemic lupus erythematosus. J Immunol 122:614

    Google Scholar 

  43. Rook AH, Tsokos GC, Quinnan GV Jr, Balow JE, Ramsey KM, Stocks N, Phelan MA, Djeu JY (1982) Cytotoxic antibodies to natural killer cells in systemic lupus erythematosus. Clin Immunol Immunopathol 24:179

    Google Scholar 

  44. Sakane T, Steinberg AD, Reeves JP, Green I (1979) Studies of immune functions of patients with systemic lupus erythematosus. J Clin Invest 63:954

    Google Scholar 

  45. Sakane T, Steinberg AD, Reeves JP, Green I (1979) Studies of immune functions of patients with systemic lupus erythematosus: T-cell subsets and antibodies to T-cell subsets. J Clin Invest 64:1260

    Google Scholar 

  46. Sakane T, Kotani H, Takada S, Murakawa Y, Ueda Y (1983) A defect in the suppressor circuits among OKT4+ cell populations in patients with systemic lupus erythematosus occurs independently of a defect in the OKT8+ suppressor T cell function. J Immunol 131:753

    Google Scholar 

  47. Steinberg AD, Klassen LW, Budman DR, Williams GW (1979) Immunofluorescence studies of anti-T cell antibodies and T cells in systemic lupus erythematosus. Selective loss of brightly staining cells in active disease. Arthritis Rheum 22:114

    Google Scholar 

  48. Takahashi H, Terasaki PI, Iwaki Y, Nakata S (1980) Identification of surface IgM as the target antigen of cold lymphocytotoxins. Tissue Antigens 16:176

    Google Scholar 

  49. Takeuchi T, Abe T, Kiyotaki M, Toguchi T, Koide J, Morimoto C, Homma M (1982) In vitro immune response of SLE lymphocytes. The mechanism involved in B-cell activation. Scand J Immunol 16:369

    Google Scholar 

  50. Takeuchi T, Rudd CE, Schlossman SF, Morimoto C (1987) Induction of suppression following autologous mixed lymphocyte reaction; role of a novel 2H4 antigen. Eur J Immunol 17:97

    Google Scholar 

  51. Takeuchi T, Tanaka S, Steinberg AD, Matsuyama T, Daley J, Schlossman SF, Morimoto C (1988) Defective Expression of the 2H4 Molecule after autologous mixed lymphocyte reaction activation in systemic lupus erythematosus patients. J Clin Invest 82:1288

    Google Scholar 

  52. Tanaka S, Matsuyama T, Steinberg AD, Schlossman SF, Morimoto C (1989) Antilymphocyte antibodies against CD4+2H4+ cell populations in patients with systemic lupus erythematosus. Arthritis Rheum 32:398

    Google Scholar 

  53. Terasaki PI, McClelland JD (1964) Microdroplet assay of human serum cytotoxins. Nature 204:998

    Google Scholar 

  54. Terasaki PI, Mottironi VD, Barnett EV (1970) Cytotoxins in disease. Autocytotoxins in lupus. N Engl J Med 283:724

    Google Scholar 

  55. Thomas DB (1973) Antibodies specific for human T lymphocytes in cold agglutinin and lymphocytotoxic sera. Eur J Immunol 3:824

    Google Scholar 

  56. Thomas ML (1989) The leukocyte common antigen family. Annu Rev Immunol 7:339

    Google Scholar 

  57. Tonks NK, Charbonneau H, Diltz CD, Fischer EH, Walsh KA (1988) Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase. Biochemistry 27:8695

    Google Scholar 

  58. Twomey JJ, Laughter AH, Steinberg AD (1978) A serum inhibitor of immune regulation in patients with systemic lupus erythematosus (SLE). J Clin Invest 62:713

    Google Scholar 

  59. Williams RC Jr, Lies RB, Messner RP (1973) Inhibition of mixed leukocyte culture responses by serum and τ-globulin fractions from certain patients with connective tisue disorders. Arthritis Rheum 16:597

    Google Scholar 

  60. Williams RC Jr, Bankhurst AD, Montaño JD (1976) IgG anti-lymphocyte antibodies in SLE detected by 125I protein A. Arthritis Rheum 19:1261

    Google Scholar 

  61. Winfield JB, Winchester RJ, Kunkel HG (1975) Association of cold-reactive anti-lymphocyte antibodies with lymphopenia in systemic lupus erythematosus. Arthritis Rheum 18:587

    Google Scholar 

  62. Winfield JB, Winchester RJ, Wernet P, Fu SM, Kunkel HG (1975) Nature of cold-reactive antibodies to lymphocyte surface determinants in systemic lupus erythematosus. Arthritis Rheum 18:1

    Google Scholar 

  63. Winfield JB, Shaw M, Minota S (1986) Modulation of IgM anti-lymphocyte antibody-reactive T cell surface antigens in systemic lupus erythematosus. J Immunol 136:3246

    Google Scholar 

  64. Winfield JB, Shaw M, Yamada A, Minota S (1987) Subset specificity of anti-lymphocyte antibodies in systemic lupus erythematosus. II. Preferential reactivity with T4+ cells is associated with relative depletion of autologous T4+ cells. Arthritis Rheum 30:162

    Google Scholar 

  65. Yamada A, Winfield JB (1984) Inhibition of soluble antigen-induced T cell proliferation by warm-reactive antibodies to activated T cells in systemic lupus erythematosus. J Clin Invest 74:1948

    Google Scholar 

  66. Yamada A, Cohen PL, Winfield JB (1985) Subset specificity of anti-lymphocyte antibodies in systemic lupus erythematosus. Preferential reactivity with cells bearing the T4 and autologous erythrocyte receptor phenotypes. Arthritis Rheum 28:262

    Google Scholar 

  67. Yamada A, Shaw M, Winfield JB (1985) Surface antigen specificity of cold-reactive IgM antilymphocyte antibodies in systemic lupus erythematosus. Arthritis Rheum 28:44

    Google Scholar 

  68. Yamada A, Minota S, Nojima Y, Yazaki Y (1993) Changes in subset specificity of anti-T cell autoantibodies in systemic lupus erythematosus. Autoimmunity 14:269

    Google Scholar 

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Authors and Affiliations

  1. Division of Rheumatology and Immunology, University of North Carolina, Chapel Hill, 932 FLOB 231H CB#7280, 27599, Chapel Hill, NC, USA

    John B. Winfield, Philip Fernsten & Jan Czyzyk

  2. University of Arizona, 85724, Tucson, Arizona, USA

    Ena Wang & John Marchalonis

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Winfield, J.B., Fernsten, P., Czyzyk, J. et al. Antibodies to CD45 and other cell membrane antigens in systemic lupus erythematosus. Springer Semin Immunopathol 16, 201–210 (1994). https://doi.org/10.1007/BF00197517

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