Abstract
The present study describes the changes in the endocrine pancreas of severely diabetic calmodulin-transgenic mice using light microscopic immunocyto-chemical and morphometric techniques. A marked reduction in the number and volume of islets, together with distortion of their normal architecture, was found in diabetic mice. In addition, the volume density of both endocrine tissue and B-cells was decreased. An irregular distribution of non-B-cells was also observed in diabetic animals. The volume density and the percentage of A-cells appeared increased. However, when quantified per area unit, the number of all the islet cell types diminished, although only the decrease in B-cell number was statistically significant. The decrease in B-cell mass might account for the diabetic state developed in this animal model.
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Baetens D, Stephan Y, Ravazzola M, Malaisse-Lagae F, Coleman DL, Orci L (1978) Alteration of islet cell populations in spontaneously diabetic mice. Diabetes 27:1–7
Bonner-Weir S (1988) Morphological evidence for pancreatic polarity of β-cell within islets of Langerhans. Diabetes 37:616–621
Epstein PN, Overbeek PA, Means AR (1989) Calmodulin-induced early-onset diabetes in transgenic mice. Cell 58:1067–1073
Gepts W, Lecompte PM (1981) The pancreatic islets in diabetes. Am J Med 70:105–115
Klöppel G, Gepts W, Veld PA (1992) Morphology of the pancreas in normal and diabetic states. In: Alberti KGMM, de Fronzo RA; Keen H, Zimmet P (eds) International textbook of diabetes mellitus. John Wiley and Sons, Chichester
Leiter EH, Graph DA, Eppig JJ, Coleman DL (1979) Ultrastructural and morphometric studies of delta cells in pancreatic islets from C57 BL/Ks diabetic mice. Diabetologia 17:297–309
Matsushima Y, Makino H, Kanatsuka A, Osegawa M, Kumagai A, Nishimura M, Toshino Y, Makino S (1982) Pancreatic somatostatin contents in spontaneously diabetic KK and non obese diabetic (NOD) mice. Horm Metab Res 14:292–298
McEvoy RC, Hegre OD (1977) Morphometric quantitation of the pancreatic insulin-, glucagon-, and somatostatin-positive cell population in normal and alloxan-diabetic rats. Diabetes 26:1140–1146
Orci L, Baetens D, Rufener C, Amherdt M, Ravazzola M, Studer P, Malaisse-Lagae F, Unger RH (1976) Hypertrophy and hyperplasia of somatostatin-containing D-cells in diabetes. Proc Natl Acad Sci USA 73:1338–1342
Petersson B, Elde R, Efendic S, Hökfelt T, Johansson O, Luft R, Cerasi E, Hellerström C (1977) Somatostatin in the pancreas, stomach and hypothalamus of the diabetic Chinese hamster. Diabetologia 13:463–467
Rahier J, Goebels RM, Henquin JC (1983) Cellular composition of the human diabetic pancreas. Diabetologia 24:366–371
Reaven EP, Gold G, Reaven GM (1979) Effect of age on glucose-stimulated insulin release by the beta cell of the rat. J Clin Invest 64:591–599
Shafrir E (1990) Diabetes in animals. In: Rifkin H, Porte D Jr (eds) Diabetes mellitus, 4th edn. Elsevier, New York, pp 299–340
Stefan Y, Orci L, Malaisse-Lagae F, Perrelet A, Patel Y, Unger RH (1982) Quantitation of the endocrine cell content in the pancreas of nondiabetic and diabetic humans. Diabetes 31:694–700
Sternberger LA, Handy PH, Cuculis JJ, Meyer HC (1970) An unlabelled antibody method of immunocytochemistry. J Histochem Cytochem 18:315–340
Tannenbaum GS, Colle E, Wanamaker L, Gurd W, Goldman H, Seemayer TA (1981) Dynamic time-course studies of the spontaneously diabetic BB Wistar rat. II. Insulin-, glucagon-, and somatostatin-reactive cells in the pancreas. Endocrinology 109:1880–1887
Unger RH, Orci L (1977) Possible roles of the pancreatic D-cell in the normal and diabetic states. Diabetes 26:241–244
Van Asche FA, Gepts W, Aerts L (1980) Immunocytochemical study of the endocrine pancreas in the rat during normal pregnancy and during experimental diabetic pregnancy. Diabetologia 18:487–491
Weibel ER (1969) Stereological principles for morphometry in electron-microscopic cytology. Int Rev Cytol 26:235–302
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Gómez Dumm, C.L.A., Gagliardino, J.J., Atwater, I. et al. Quantitative immunocytochemical study of islet cell populations in diabetic calmodulin-transgenic mice. Vichows Archiv A Pathol Anat 425, 73–77 (1994). https://doi.org/10.1007/BF00193952
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DOI: https://doi.org/10.1007/BF00193952