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Chromosomal localization, genomic structure, and allelic polymorphism of the human CD79a (lg-αmb-1) gene

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Abstract

The germline DNA sequence of the human CD79a (Ig-α/mb-1) gene was determined by polymerase chain reaction sequencing of a cosmid clone derived from an arrayed human chromosome 19 library .The CD79a gene was localized to chromosome 19q13.2; this localization places the gen within the CEA-like gene cluster with the following gene order: -CEA-CGMI-CD79a-RPS11-ATPIA3-BGP-CGM9-. The genomic organization of the human CD79a gene resembles the mousse counterpart with five exons interrupted by four introns. Computer analyszes suggest the presence of transcription regulatory elements known to be important in the regulation of mouse CD79a (AP-1, EBF, AP-2, MUF2, and SP-1 sites), as well as elements not found in the mouse gene (an NF-κB binding site and a series of E-box motifs). Similar to the mouse gene, the5′ flanking region of human CD79a lacks a TATA box; however, unlike mouse CD79a, a classical octamer motif could not be identified in the human gene. Finally, a new Rsa restriction fragment length polymorphism was defined in the non-coding regions of the human gene.

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  1. Shiori Hashimoto

    Present address: Departement of Neurology, Tokyo Women's Medical College, 10 Kawada-Cho, Shinjuku-Ku, Tokyo, Japan

Authors and Affiliations

  1. Department of Medicine, North Shore University Hospital, 350 Community Drive, 11030, Manhasset, NY, USA

    Shiori Hashimoto, Peter K. Gregersen & Nicholas Chiorazzi

  2. Cornell University Medical College, 10021, New York, NY, USA

    Shiori Hashimoto, Peter K. Gregersen & Nicholas Chiorazzi

  3. Human Genome Center, Biology and Biotechnology Research Program, The Lawrence Livermore National Laboratory, 94550, Livermore, CA, USA

    Haarvey Mohrenweiser

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  1. Shiori Hashimoto
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Hashimoto, S., Mohrenweiser, H., Gregersen, P.K. et al. Chromosomal localization, genomic structure, and allelic polymorphism of the human CD79a (lg-αmb-1) gene. Immunogenetics 40, 287–295 (1994). https://doi.org/10.1007/BF00189974

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  • DOI: https://doi.org/10.1007/BF00189974

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