Summary
In a time course study (4–20 h) rabbit early cleavage stages (day 1 p.c.) and compacted morulae (day 3 p.c.) were exposed to visible light or room temperature (23° C), respectively. An 8 h light exposure of day 1 embryos caused alterations in nuclear morphology (lobulated nuclei, loss of nucleolar diffentiation), an increased electron density of the cytoplasm, and cellular fragmentation leading to a considerable degeneration of blastomeres (central clustering of organelles, loss of cell surface differentiation) after a 20 h exposure. Room temperature exposure (compacted Day 3 morulae) led to decompaction and a cleavage delay after 8 h. After 10 h, arrested metaphases occurred in all examined morulae. Even after 20 h at 23° C, day 3 embryos were at the decompacted morula stage, and showed metaphase-arrested blastomeres. The general morphology of the blastomeres was unaffected at this temperature, except for vacuolated serand cis-side vesicles of the Golgi complex at 8, 12 and 20 h, respectively.
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References
Anderson E, Condon W, Sharp D (1971) A study of oogenesis and early embryogenesis in the rabbit, Oryctolagus cuniculus, with special reference to the structural changes of mitochondria. J Morphol 130:67–92
Bavister BD (1987) Studies on the developmental blocks in cultured hamster embryos. In: Bavister BD (ed) The mammalian preimplantation embryo. Regulation of growth and differentiation in vitro. Plenum Press, New York, pp 219–249
Bavister BD (1988) Role of oviductal secretions in embryonic growth in vivo and in vitro. Theriogenology 29:143–154
Chang MC (1948) The effects of low temperature on fertilized rabbit ova in vitro, and the normal development of ova kept at low temperature for several days. J Gen Physiol 31:385–409
Clarke PGH (1990) Developmental cell death: morphological diversity and multiple mechanisms. Anat Embryol 181:195–213
Danielsen EM, Hansen GH, Cowell GM (1989) Biosynthesis of intestinal microvillar proteins. Low temperature arrests both processing and intracellular transport. Eur J Cell Biol 49:123–127
Fischer B (1987) Development retardation in cultured preimplantation rabbit embryos. J Reprod Fertil 79:115–123
Fischer B (1988) Embryonalentwicklung in vitro und in vivo. Charakterisierung entwicklungsspezifischer Strukturen und Lebensäußerungen von Präimplantationsembryonen in vitro und in vivo. Copythek, Enke, Stuttgart
Fischer B (1989) Effects of asynchrony on rabbit blastocyst development. J Reprod Fertil 86:479–491
Fischer B, Meuser-Odenkirchen G (1988) A 2-year follow-up of effects of biotechniques on reproduction in the domestic rabbit, Oryctolagus cuniculus. Lab Anim 22:5–15
Fischer B, Schumacher A, Hegele-Hartung C, Beier HM (1988) Potential risk of light and room temperature exposure to preimplantation embryos. Fertil Steril 50:938–944
Hegele-Hartung C, Fischer B, Beier HM (1988a) Development of preimplantation rabbit embryos after in vitro culture and embryo transfer: an electron microscopic study. Anat Rec 220:31–42
Hegele-Hartung C, Schumacher A, Fischer B (1988b) Ultrastructure of preimplantation rabbit embryos exposed to visible light and room temperature. Anat Embryol 178:229–241
Hegele-Hartung C, Piegsa K, Fischer B (1989) Effect of osmolarity of the fixative on the ultrastructure of preimplantation rabbit embryos. Acta Anat 136:79–88
Hesseldahl H (1971) Ultrastructure of early cleavage stages and preimplantation in the rabbit. Z Anat Entwicklungsgesch 135:139–135
Inoué S (1981) Cell division and the mitotic spindle. J Cell Biol 91:131s-147s
Kane MT (1987a) Culture media and culture of early embryos. Theriogenology 27:49–57
Kane MT (1987b) In vitro growth of preimplantation rabbit embryos. In: Bavister BD (ed) The mammalian preimplantation embryo. Plenum Press, New York, pp 193–217
Kane MT, Foote RH (1970) Culture of two-and four-cell rabbit embryos to the expanding blastocyst stage in synthetic media. Proc Soc Exp Biol Med 133:921–925
Kane MT, Foote RH (1971) Factors affecting blastocyst expansion of rabbit zygotes and young embryos in defined media. Biol Reprod 4:41–47
Karp LE, Smith WD (1975) Experimental production of aneuploidy in mouse oocytes. Gynecol Invest 6:337–341
Kerr JFR, Bishop CJ, Searly J (1984) Apoptosis. Recent Adv Histopathol 12:1–15
King WA, Niar A, Chartrain I, Betteridge KJ, Guay P (1988) Nucleolus organizer regions and nucleoli in preattachment bovine embryos. I Reprod Fertil 82:87–95
Lambert AM, Bajer AS (1977) Microtubule distribution and reversible arrest of chromosome movements induced by low temperature. Cytobiologie 15:1–23
Magistrini M, Szöllösi D (1980) Effects of cold and of isopropyl-Nphenylcarbonate on the second meiotic spindle of mouse oocytes. Eur J Cell Biol 22:699–707
Manes C (1973) The participation of the embryonic genome during early cleavage in the rabbit. Dev Biol 32:453–459
Manes C (1977) Nucleic acid synthesis in preimplantation rabbit embryos. III. A “dark period” immediately following fertilization, and the early predominance low molecular weight RNA synthesis. J Exp Zool 201:247–258
Overström EW (1987) In vitro assessment of blastocyst differentiation. In: Bavister BD (ed) The mammalian preimplantation embryo. Regulation of growth and differentiation in vitro. Plenum Press, New York, pp 95–116
Pickering SJ, Johnson MH (1987) The influence of cooling on the organization of the meiotic spindle of the mouse oocyte. Hum Reprod 2:207–216
Saraste J, Kuismanen E (1984) Preand post-Golgi vacuoles operate in the transport of Semliki forest virus membrane glycoproteins to the cell surface. Cell 38:535–549
Saraste J, Palade GE, Farguhar MG (1986) Temperature-sensitive steps in the transport of secretory proteins through the Golgi complex in exocrine pancreatic cells. Proc Natl Acad Sci USA 83:6425–6429
Sathananthan AH, Trounson A, Freeman L, Brady T (1988) The effects of cooling human oocytes. Hum Reprod 3:968–977
Schumacher A, Fischer B (1988) The influence of visible light and room temperature on cell proliferation in preimplantation rabbit embryos. J Reprod Fertil 84:197–204
Schumacher A, Hegele-Hartung C, Mootz U, Beier HM, Fischer B (1989) Developmental capacity of preimplantation rabbit embryos injured by exposure to visible light or room temperature. J Reprod Fertil Abstract Series No. 3:19 (abstr. 28)
Spielmann H (1987) Analysis of embryo toxic effects in preimplantation embryos. In: Bavister BD (ed) The mammalian preimplantation embryo. Regulation of growth and differentiation in vitro. Plenum Press, New York, pp 309–331
Sundström P, Nilsson O, Liedholm P (1981) Cleavage rate and morphology of early human embryos obtained after artificial fertilization and culture. Acta Obstet Gynecol Scand 60:109–120
Trounson A, Sathananthan AH (1984) The application of electron microscopy in the evaluation of two-to four-cell human embryos cultured in vitro for embryo transfer. J In Vitro Fert Embryo Transfer 1:153–165
Van Blerkom J, Manes C, Daniel JC Jr (1973) Development of preimplantation rabbit embryos in vivo and in vitro. Dev Biol 35:262–282
Wyllie AH (1987) Apoptosis. Cell death under homeostatic control. Arch Toxicol [Suppl] 11:3–10
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Hegele-Hartung, C., Schumacher, A. & Fischer, B. Effects of visible light and room temperature on the ultrastructure of preimplantation rabbit embryos: a time course study. Anat Embryol 183, 559–571 (1991). https://doi.org/10.1007/BF00187905
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DOI: https://doi.org/10.1007/BF00187905