Abstract
Anaerobic granules developed for the treatment of pentachlorophenol (PCP) completely minearilized14C-labeled PCP to14CH4 and14CO2. Release of chloride ions from PCP was performed by live cells in the granules under anaerobic conditions. No chloride ions were released under aerobic conditions or by autoclaved cells. Addition of sulfate enhanced the initial chloride release rate and accelerated the process of mineralization of14C-labeled PCP. Addition of molybdate (10 mM) inhibited the chloride release rate and severely inhibited PCP mineralization. This suggests involvement of sulfate-reducing bacteria in PCP dechlorination and mineralization. Addition of 2-bromoethane sulfonate slightly decreased the chloride release rate and completely stopped production of14CH4 and14CO2 from [14C]PCP. 2,4,6-trichlorophenol was observed as an intermediate during PCP dechlorination. On the basis of experimental results, dechlorination of 2,4,6-trichlorophanol by the granules was conducted through 2,4-dichlorophenol, 4-chlorophenol or 2-chlorophenol to phenol at pH 7.0–7.2.
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Dedicated to the memory of Dr. L. Bhatnagar, who died before the manuscript was completed
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Kennes, C., Wu, W.M., Bhatnagar, L. et al. Anaerobic dechlorination and mineralization of pentachlorophenol and 2,4,6-trichlorophenol by methanogenic pentachlorophenol-degrading granules. Appl Microbiol Biotechnol 44, 801–806 (1996). https://doi.org/10.1007/BF00178622
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DOI: https://doi.org/10.1007/BF00178622