Summary
Experiments were designed to study the interaction between prejunctional α2-adrenoceptors and both adenosine and opioid receptors at the postganglionic sympathetic nerve endings innervating the tail artery of the rat. Segments of this vessel were preincubated with [3H]-noradrenaline and then perfused/superfused with [3H]-noradrenaline-free medium. Their perivascular nerves were field stimulated with standard stimulation parameters: 24 pulses at 0.4 Hz, 0.3 ms, 200 mA. In some experiments, the stimulation parameters were adjusted in order to obtain similar reference release values despite the presence of a first release-modulating drug.
The adenosine agonist 5′-N-ethylcarboxamidoadenosine (NECA; 0.3–10 μmol/l) and [D-Ala2,MePhe4,Glyol5]enkephalin (DAGO; 0.3–10 μmol/l) depressed the stimulation-evoked overflow of tritium in a concentration dependent manner. The release-inhibiting effect of both NECA and DAGO was enhanced in the presence of the α2-adrenoceptor antagonist rauwolscine (3 μmol/l) while it was attenuated in the presence of the α2-adrenoceptor agonist 5-bromo-6-[2-imidazolin-2yl-aminol-quinoxaline (UK-14,304; 0.1 μmol/l). These changes occurred both at standard and adjusted stimulation parameters.
These results demonstrate that the prejunctional adenosine A1- and opioid μ-receptors interact with the prejunctional α2-adrenoceptors. The level at which these interactions take place (receptors themselves or transduction mechanisms) as well as the physiological significance of the phenomenon remain to be determined.
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Bucher, B., Corriu, C. & Stoclet, J.C. Prejunctional opioid μ-receptors and adenosine A1-receptors on the sympathetic nerve endings of the rat tail artery interact with the α2-adrenoceptors. Naunyn-Schmiedeberg's Arch Pharmacol 345, 37–43 (1992). https://doi.org/10.1007/BF00175467
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DOI: https://doi.org/10.1007/BF00175467