Summary
The effect of culture conditions, especially nitrogen/carbon (N/C) ratio and temperature, on acyl lipid profiles in the oleaginous yeast Rhodotorula glutinis NRRL Y 1091 have been investigated. Cultivation of the microorganism under N-limited conditions (N/C < 0.14 g/g) resulted in enhanced fatty acid (FA) cell content but a reduced relative amount of polyunsaturated fatty acids (PUFA). The maximal FA productivity was obtained for a 0.025 N/C ratio resulting from the arrangement between the specific rate of FA synthesis and the concentration of lipid-free biomass. Under nitrogen-non-limiting conditions, cells grown at lower temperatures had a higher PUFA content and the maximal productivity of α-linolenic acid was obtained by shifting the temperature of the culture from 30° C to 25° C.
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Granger, L.M., Perlot, P., Goma, G. et al. Kinetics of growth and fatty acid production of Rhodotorula glutinis . Appl Microbiol Biotechnol 37, 13–17 (1992). https://doi.org/10.1007/BF00174195
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DOI: https://doi.org/10.1007/BF00174195