Summary
At pH 3.6, Lactobacillus plantarum is unable to grow on citrate or to ferment it in the absence of another carbon source such as glucose. In a defined medium containing glucose and citrate, with a higher concentration of the former than the latter, as in many fermented alcoholic beverages, L. plantarum will first ferment the sugar. The production of lactate from glucose degradation increases the acidity of the medium and inhibits the fermentation of citrate. In co-culture with Saccharomyces cerevisiae, part of the glucose is fermented by the yeast, partly avoiding the pH drop and the inhibition of citrate fermentation by L. plantarum. Fermentation was still possible at pH values around 3.0.
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References
Amerine MA, Kunkee RE (1968) Microbiology of winemaking. Annu Rev Microbiol 22:323–358
Cogan TM (1987) Co-metabolism of citrate and glucose by Leuconostoc spp: effects on growth, substrates and products. J Appl Bacteriol 63:551–558
Davis CR, Wibowo DJ, Lee TH, Fleet GH (1986) Growth and metabolism of lactic acid bacteria during and after malolactic fermentation of wines at different pH. Appl Environ Microbiol 51:539–543
De Man JC, Rogosa M, Sharpe ME (1960) A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135
De Vuyst A, Vervack W, Vanbelle M, Moreels A, Arnould R (1965) Comparaison du dosage des acides gras volatils dans les ensilages et le liquide du rumen par la methode de Leppe-Fleig. Agricultura 12:223–236
Drysdale GS, Fleet GH (1989) The effect of acetic acid bacteria upon the growth and metabolism of yeasts during the fermentation of grape juice. J Appl Bacteriol 67:471–481
Fleet GH (1990) Yeasts in dairy products. J Appl Bacteriol 68:199–221
Gutmann I, Wahlefeld AN (1974) Laevo-lactate Bestimmung mit Lactat-dehydrogenase und NAD. In: Bergmeyer HH (ed) Methoden der enzymatischen Analyse, vol 2. Verlag Chemie, Weinheim, pp 1510–1514
Kandler O, Weiss N (1986) Regular, nonsporing Gram-positive rods. In: Sneath PHA (ed) Bergey's manual of systematic bacteriology vol 2. Williams and Wilkins, Baltimore, pp 1208–1260
Kennes C, Veiga MC, Dubourguier HC, Touzel JP, Albagnac G, Naveau H, Nyns EJ (1991) Trophic relationships between Saccharomyces cerevisiae and Lactobacillus plantarum and their metabolism of glucose and citrate. Appl Environ Microbiol 57:1046–1051
Lodder J (1970) The yeasts, a taxonomic study. North-Holland Publishing, Amsterdam
McDonald LC, Fleming HP, Hassan HM (1990) Acid tolerance of Leuconostoc mesenteroides and Lactobacillus plantarum. Appl Environ Microbiol 56:2120–2124
Pauss A, Monzambe K, Kennes C, Naveau H, Nyns EJ (1988) Can durable continuous fermentations be conducted under non-sterile conditions? In: Kyslic P, Dawes EA, Krumphanzl V, Novak M (eds) Continuous culture. Academic Press, London, pp 57–70
Puhan Z, Vogt O (1985) Hefehaltige Sauermilchprodukte. Technologie und Stoffwechsel. Dtsch Molk Ztg 3:68–76
Steinkraus KH (1982) Fermented foods and beverages: the role of mixed cultures. In: Bushell ME, Slater JH (eds) Microbial interactions and communities, vol 1. Academic Press, London, pp 422–433
Zweig G, Sherna J (1972) Handbook of chromatography, CRC Press, Cleveland, Ohio, p 130
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Kennes, C., Dubourguier, H.C., Albagnac, G. et al. Fermentation of citrate by Lactobacillus plantarum in the presence of a yeast under acid conditions. Appl Microbiol Biotechnol 35, 369–372 (1991). https://doi.org/10.1007/BF00172728
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DOI: https://doi.org/10.1007/BF00172728