Abstract
Effects of leptomeningeal tumor on the brain parenchyma was studied by the immunohistochemical method with astroprotein (GFAP) and tubulin in a rat model of meningeal carcinomatosis. Thickening of subpial glial lining (external glial layer) and hypertrophy of subpial astrocytes, detected by the antiserum to GFAP, was the early sign of parenchymal involvement. The glial lining was continuous as far as the tumor cells were confined to the subarachnoid space, however, penetration of tumor cells into subpial brain was associated with disruption of the glial lining. Speculative role of this lining in preventing the tumor cell to infiltrate into brain tissue was discussed. In contrast to the prominent immunohistochemical changes in astrocytes, neuronal tubulin immunoreactivity was not altered even in the late stage of the disease. The present study demonstrated that the leptomeningeal dissemination of tumor cells did cause pathologic change in brain parenchyma as was evidenced by the reactive change of astrocytes. However, the preserved immunoreaction for tubulin suggested that the nerve cell damage was not severe even at the advanced stage of the disease.
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Hojo S, Hirano A: The pathology of metastasis affecting the central nervous system. In: Takakura K, Sano K, Hojo S, Hirano A (eds) Metastatic brain tumor. Igaku-Shoin, Tokyo and New York, 1981, pp 5–111
Olson ME, Chernik N, Posner JB: nfiltration of the leptomeninges by systemic cancer, a clinical and pathological study. Arch Neurol 30:122–137, 1974
Grain GO, Karr JP: Diffuse leptomeningeal carcinomatosis, clinical and pathological characteristics. Neurology (Minneap) 5:706–722, 1955
Gonzalez-Vitae JC, Garcia-Bunuel R: Meningeal carcinomatosis. Cancer 37:2906–2911, 1976
Heathfield KWG, Williams JRD: Carcinomatosis of the meninges, some clinical and pathological aspects. Brit Med J 11:328–330, 1956
Little JR, Dale AJD, Okazaki H, Minu R: Meningeal carcinomatosis, clinical manifestation. Arch Neurol 30:138–143, 1974
Yoshimine T, Ushio Y, Hayakawa T, Hasegawa T, Arita N, Yamada K, Jamshidi J, Mogami H: Immunohistochemical study of metastatic brain tumors with astroprotein (GFAP), a glia-specific protein; tissue architecture and the origin of blood vessels. J Neurosurg 62:414–418, 1985
Yoshimine T, Morimoto K, Homburger HA, Yanagihara T: Immunohistochemical localization of creatine kinase BB-isoenzyme in human brain, comparison with tubulin and astroprotein. Brain Res 265:101–108, 1983
Yoshimine T, Morimoto K, Homburger HA, Yanagihara T: Immunohistochemical localization of creatine kinase BB-isoenzyme and tubulin in gerbil brain. Neuroscience 12:959–969, 1984
Wuerker RB, Kirkpatrick JB: Neuronal microtubules, neurofilaments, and microfilaments. Int Rev Cytol 33:45–75, 1972
Ushio Y, Chernik NL, Posner JB, Shapiro WR: Meningeal carcinomatosis, development of an experimental model. J Neuropathol Exp Neurol 36:228–244, 1977
Braak E: On the fine structure of the external glial layer in the isocortex of man. Cell Tiss Res 157:367–390, 1975
Yoshimine T, Ushio Y, Hayakawa T, Arita N, Mori T: Ependymal reaction to stab wounds in rat brains, immunocytochemical study with antiserum to astroprotein. Neurol Med Chir (Tokyo) 22:19–23, 1982
Yoshimine T, Morimoto K, Brengman JM, Homburger HA, Mogami H, Yanagihara T: Immunohistochemical investigation of cerebral ischemia during recirculation. J Neurosurg 63:922–928, 1985
Yoshimine T, Ushio Y, Hayakawa T, Takemoto O, Maruno M, Mogami H: Growth activity of tumors at different intracranial structures. Immunohistochemical study with bromodeoxymidine. Acta Neuropathol (Berl) 71: 15–18, 1986.
Price RA, Johnson WW: The central nervous system in childhood leukemia. I. The arachnoid. Cancer 31:520–533, 1973
Ramsey HJ: Fine structure of the surface of the cerebral cortex of human brain. J Cell Biol 26:323–333, 1965
Lopes CAS, Mair WGP: Ultrastructure of the outer cortex and the pia mater in man. Acta Neuropathol (Berl) 28:79–86, 1974
Yanagihara T, Yoshimine T, Morimoto K, Homburger HA: Immunohistochemical investigation of cerebral ischemia in gerbils. J Neuropathol Exp Neurol 44:204–215, 1985
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Jamshidi, J., Yoshiminel, T., Ushio, Y. et al. Pathology of brain parenchyna in meningeal carcinomatosis; Immunohistochemical study with astroprotein (GFAP) and tubulin. J Neuro-Oncol 5, 65–71 (1987). https://doi.org/10.1007/BF00162767
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DOI: https://doi.org/10.1007/BF00162767