Abstract
Flash-induced redox reactions in spinach PS II core particles were investigated with absorbance difference spectroscopy in the UV-region and EPR spectroscopy. In the absence of artificial electron acceptors, electron transport was limited to a single turnover. Addition of the electron acceptors DCBQ and ferricyanide restored the characteristic period-four oscillation in the UV absorbance associated with the S-state cycle, but not the period-two oscillation indicative of the alternating appearance and disappearance of a semiquinone at the QB-site. In contrast to PS II membranes, all active centers were in state S1 after dark adaptation. The absorbance increase associated with the S-state transitions on the first two flashes, attributed to the Z+S1→ZS2 and Z+S2→ZS3 transitions, respectively, had half-times of 95 and 380 μs, similar to those reported for PS II membrane fragments. The decrease due to the Z+S3→ZS0 transition on the third flash had a half-time of 4.5 ms, as in salt-washed PS II membrane fragments. On the fourth flash a small, unresolved, increase of less than 3 μs was observed, which might be due to the Z+S0→ZS1 transition. The deactivation of the higher S-states was unusually fast and occurred within a few seconds and so was the oxidation of S0 to S1 in the dark, which had a half-time of 2–3 min. The same lifetime was found for tyrosine D+, which appeared to be formed within milliseconds after the first flash in about 10% inactive centers and after the third and later flashes by active centers in Z+S3.
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Abbreviations
- Bis-Tris:
-
(bis[2-hydroxyethyl]imino-tris[hydroxymethyl]methane)
- D:
-
secondary electron donor of PS II
- DCBQ:
-
2,5-dichloro-p-benzoquinone
- DCMU:
-
3-(3′,4′dichlorophenyl)-1,1-dimethylurea
- PS II:
-
Photosystem II
- QA :
-
secondary electron acceptor of PS II
- S0–3 :
-
redox state of the oxygen-evolving complex
- Z:
-
secondary electron donor of PS II
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van Leeuwen, P.J., Heimann, C., Gast, P. et al. Flash-induced redox changes in oxygen-evolving spinach Photosystem II core particles. Photosynth Res 38, 169–176 (1993). https://doi.org/10.1007/BF00146416
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DOI: https://doi.org/10.1007/BF00146416