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Cloning and nucleotide sequence of a cDNA encoding a major fucoxanthin-, chlorophylla/c-containing protein from the chrysophyteIsochrysis galbana: implications for evolution of thecab gene family

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Abstract

We investigated the primary structure of a cDNA encoding a light-harvesting protein from the marine chrysophyteIsochrysis galbana. Antibodies raised against the major fucoxanthin, chlorophylla/c-binding light-harvesting protein (FCP) ofI. galbana were used to select a cDNA clone encoding one of the FCP apoproteins. The nucleic acid and deduced amino acid sequences reveal conserved regions within the first and third transmembrane spans with Chla/b-binding proteins and with FCPs of another chromophyte. However, the amino acid identity betweenI. galbana FCP and othercab genes of FCPs is only ca. 30%. Phylogenetic analyses demonstrated that the FCP genes of both diatoms and chrysophytes sequenced to date are more closely related tocab genes encoding LHC I, CP 29, and CP 24 of higher plants than tocab genes encoding LHC II of chlorophytes. We propose that LHC I, CP 24 and CP 29 and FCP might have originated from a common ancestral chl binding protein and that the major LHC II of Chla/b-containing organisms arose after the divergence between the chromophytes and the chlorophytes.

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Authors and Affiliations

  1. Brookhaven National Laboratory, 11973, Upton, NY, USA

    Julie LaRoche, Kevin Wyman & Paul Falkowski

  2. Marine Sciences Research Center, State University of New York, 11794, Stony Brook, NY, USA

    Diane Henry

  3. Israel Oceanographic and Limnological Research Institute, P.O. Box 8030, 31080, Haifal, Israel

    Assaf Sukenik

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  1. Julie LaRoche
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  2. Diane Henry
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  4. Assaf Sukenik
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  5. Paul Falkowski
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LaRoche, J., Henry, D., Wyman, K. et al. Cloning and nucleotide sequence of a cDNA encoding a major fucoxanthin-, chlorophylla/c-containing protein from the chrysophyteIsochrysis galbana: implications for evolution of thecab gene family. Plant Mol Biol 25, 355–368 (1994). https://doi.org/10.1007/BF00043865

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