Abstract
Rice cytosolic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is composed of two subunits of different molecular weights. Cytosolic GAPDH activity and protein both decreased immediately after transfer of 48-h rice seedlings to anaerobic conditions. Subsequent increase in activity and protein was accompanied by a change in isoenzyme profile and was preceded by an increase in steady-state messenger levels. One and two-dimensional electrophoretic analyses of in vivo and in vitro labeled GAPDH suggested that the change in isoenzyme profile under anaerobic conditions is due to preferential synthesis of one of the two GAPDH subunits caused by a specific increase in its mRNA.
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References
Bomsel JL, Pradet A: Energy metabolism in plants under hypoxia and anoxia. In: Hook DD, Crawford RMM (eds) Plant Life in Anaerobic Environments, pp. 89–118. Ann Arbor Science, Ann Arbor, MI (1980).
Pradet A, Mocquot B, Raymond P, Morisset C, Aspart L, Delseny M: Energy metabolism and synthesis of nucleic acids and proteins under anoxic stress. In: Key JL, Kosuge T (eds_) Cellular and Molecular Biology of Plant Stress Vol. 22, pp. 227–245. Alan R. Liss, New York (1985).
Cobb BG, Kennedy RA: Distribution of alcohol dehydrogenase in roots and shoots of rice (Oryza sativa) and Echinochloa seedlings. Plant Cell Envir 10: 633–638 (1987).
Freeling M, Bennett DC: Maize Adhl. Annu Rev Genet 19: 297–323 (1985).
Mocquot B, Pradet A, Litvak S: DNA synthesis and anoxia in rice coleoptiles. Plant Sci Lett 9: 365–371 (1977).
Aspart L, Got A, Delseny M, Mocquot B, Pradet A: Adaptation of ribonucleic acid metabolism to anoxia in rice embryos. Plant Physiol 72: 115–121 (1983).
Mocquot B, Prat C, Mouches C, Pradet A: Effect of anoxia on energy charge and protein synthesis in rice embryo. Plant Physiol 68: 636–640 (1981).
Mocquot B, Ricard B, Pradet A: Rice embryos can express heat shock genes under anoxia Biochimie 69: 677–681 (1987).
Rivoal J, Ricard B, Pradet A: Glycolytic and fermentative enzyme induction during anaerobiosis in rice seedlings. Plant Physiol Biochem (in press).
Ricard B, Mocquot B, Fournier A, Delseny M, Pradet A: Expression of alcohol dehydrogenase in rice embryos under anoxia. Plant Mol Biol 7: 321–329 (1986).
Cerff R: Evolutionary divergence of chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenases from angiosperms Eur J Biochem 126: 513–515 (1982).
Cerff R: Quaternary structure of higher plant glycealdehyde-3-phosphate dehydrogenases. Eur J Biochem 94: 243–247 (1979).
Cerff R: Separation and purification of NAD- and NADP-linked glyceraldehyde-3-phosphate dehydrogenases from higher plants. In: Edelman etal. (eds) Methods in Chloroplast Molecular Biology, pp. 683–694. Elsevier Biomedical Press, Amsterdam (1982).
Brewer GJ: An Introduction to Isozyme Techniques. Academic Press, New York (1970).
Yarbrough PO, Hecht RM: Two isoenzymes of glyceraldehyde-3-phosphate dehydrogenase in Caenorhabditis elegans. J Biol Chem 259: 14711–14720 (1984).
Kopczynski CC, Scandalios JC: Cat-2 gene expression. Developmental control of translatable CAT-2 mRNA levels in maize scutellum. Mol Gen Genet 203: 185–188 (1986).
O'Farrell PZ, Goodman HH, O'Farrell PH: High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12: 1133–1142 (1977).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Brinkman H, Martinez P, Quigley F, Martin W, Cerff R: Endosymbiotic origin and codon bias of the nuclear gene for chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize. J Mol Evol 26: 320–328 (1987).
Cerff R, Chambers SE: Subunit structure of higher plant glyceraldehyde-3-phosphate dehydrogenases (EC 1.2.1.12 and EC 1.2.1.13). J Biol Chem 254: 6094–6098 (1979).
Walker JC, Howard EA, Dennis ES, Peacock WJ: DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene. Proc Natl Acad Sci USA 84: 6624–6628 (1987).
Springer B, Werr W, Starlinger P, Bennett DC, Zokolica M, Freeling M: The shrunken gene on chromosome 9 of Zea mays L. is expressed in various plant tissues and encodes an anaerobic protein. Mol Gen Genet 205: 461–468 (1986).
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Ricard, B., Rivoal, J. & Pradet, A. Rice cytosolic glyceraldehyde 3-phosphate dehydrogenase contains two subunits differentially regulated by anaerobiosis. Plant Mol Biol 12, 131–139 (1989). https://doi.org/10.1007/BF00020498
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DOI: https://doi.org/10.1007/BF00020498