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Cortisol treatment improves the development of hypoosmoregulatory mechanisms in the euryhaline rainbow trout, Salmo gairdneri

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Abstract

The effect of cortisol on osmoregulatory parameters was studied in rainbow trout, (Salmo gairdneri), kept in freshwater (FW) and/or transferred to seawater (SW). Repeated injections of 20 µg cortisol/g fish stimulated gill and gut Na+/K+-ATPase activity and reduced plasma Na+ and Cl− levels after 2 weeks of treatment in FW-adapted fish. Cortisol doses of 0.05 and 1.0 µg/g were without effect. Repeated injections of 10 µg cortisol/g stimulated gill Na+/K+-ATPase activity and reduced plasma Na+ and Cl− levels in fish in FW, and significantly improved ion regulation after their transfer to 28SW. Higher doses of cortisol (10 and 20 µg/g) induced hyperglycemia, whereas low doses (0.05 and 1.0 µg/g were without effect or induced hypoglycemia. Plasma glucose levels decreased in cortisol-treated fish transferred to SW, whereas transient hyperglycemia was seen in the control fish.

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References cited

  • Alexis, M.N., Papaparaskeva-Papoutsoglou, E. and Papoutsoglou, S. 1984. Influence of acclimation temperature on the osmotic regulation and survival of rainbow trout (Salmo gairdneri) rapidly transferred from fresh water to sea water. Aquaculture 40: 333–341.

    Article  Google Scholar 

  • Assem, H. and Hanke, W. 1979. Concentration of carbohydrates during osmotic adjustment of the euryhaline teleost, Tilapia mossambica. Comp. Biochem. Physiol. 64A: 5–16.

    Article  Google Scholar 

  • Assem, H. and Hanke, W. 1981. Cortisol and osmotic adjustment of the euryhaline teleost, Sarotherodon mossambicus. Gen. Comp. Endocrinol. 43: 370–380.

    Article  PubMed  CAS  Google Scholar 

  • Butler, D.G. and Carmichael, F.J. 1972. (Na+-K+)-ATPase activity in eel (Anquilla rostrata) gills in relation to changes in environmental salinity: Role of adrenocortical steroids. Gen. Comp. Endocrinol. 19: 421–427.

    Article  PubMed  CAS  Google Scholar 

  • Chan, D.K.O., Chester Jones, I., Henderson, I.W. and Rankin, J.C. 1967. Studies on the experimental alteration of water and electrolyte composition of the eel (Anquilla anquilla L.). J. Endocrinol. 37: 297–317.

    PubMed  CAS  Google Scholar 

  • Cleland, K.W. and Slater, E.C. 1953. Biuret method for protein concentration. Biochem. J. 53, 547.

    PubMed  CAS  Google Scholar 

  • Dangé, A.D. 1986. Branchial Na+/K+-ATPase activity in freshwater or saltwater acclimated Tilapia Oreochromis (Sarotherodon) mossambicus: Effects of cortisol and thyroxine. Gen. Comp. Endocrinol. 62: 341–343.

    Article  PubMed  Google Scholar 

  • Doyle, W.L. and Epstein, F.H. 1972. Effects of cortisol treatment and osmotic adaptation on the chloride cells in the eel, Anquilla rostrata. Cytobiologie 6: 58–72.

    Google Scholar 

  • Eib, D. and Hossner, K.L. 1985. The effects of cortisol and actinomycin D injections on chloride cells and branchial Na+K+-ATPase in rainbow trout (Salmo gairdnert). Gen. Comp. Endocrinol. 59: 449–452.

    Article  PubMed  CAS  Google Scholar 

  • Epstein, F.H., Cynamon, M. and McKay, W. 1971. Endocrine control of Na-K-ATPase in salwater adaptation in Anquilla rostrata. Gen. Comp. Endocrinol. 16: 323–328.

    Article  CAS  Google Scholar 

  • Giles, M.A. and Vanstone, W.E. 1976. Changes in ouabain-sensitive adenosine triphosphatase activity in gills of coho salmon (Oncorhynchus kisutch) during parr-smolt transformation. J. Fish. Res. Bd. Can. 33: 54–62.

    CAS  Google Scholar 

  • Gorbman, A., Dickhoff, W.W., Vigna, S.R., Clark, N.B. and Ralph, C.L. 1983. Comparative Endocrinology. Wiley, New York.

    Google Scholar 

  • Hendersen, I.W. and Chester Jones, I. 1967. Endocrine influences on the net extrarenal fluxes of sodium and potassium in the European eel (anquilla anquilla L.) J. Endocrinol. 37: 319–325.

    Article  Google Scholar 

  • Hegab, S.A. and Hanke, W. 1984. The significance of cortisol for osmoregulation in carp (Cyprinus carpio) and tilapia (Sarotherodon mossambicus). Gen. Comp. Endocrinol. 54: 409–417.

    Article  CAS  Google Scholar 

  • Hegab, S.A. and Hanke, W. 1986. Electrolyte changes, cell volume regulation and hormonal influences during acclimation of rainbow trout (Salmo gairdneri) to salt water. Comp. Biochem. Physiol. 83A: 47–52.

    Article  Google Scholar 

  • Hirano, T. and Utida, S. 1971. Plasma cortisol concentration and the rate of intestinal water absorbtion in the eel, Anquilla japonica. Endocrinol Jap. 18: 47–52.

    CAS  Google Scholar 

  • Holmes, W.N. 1959. Studies on the hormonal control of sodium metabolism in the rainbow trout (Salmo gairdneri). Acta Endocrinol. 31: 587–602.

    PubMed  CAS  Google Scholar 

  • Jackson, A.J. 1981. Osmotic regulation in rainbow trout (Salmo gairdneri) following transfer to sea water. Aquaculture 24: 143–151.

    Article  Google Scholar 

  • Johnson, S.L., Ewing, R.D. and Lichatowich, J.A. 1977. Characterization of gill (Na+K+)-activated Adenosinetriphosphatase from chinook salmon, Oncorhynchus tshawytscha. J. Exp. Zool. 199: 345–354.

    Article  PubMed  CAS  Google Scholar 

  • Kamiya, M. 1972. Hormonal effect on Na-K-ATPase activity in the gill of Japanese eel, Anquilla japonica, with special reference to seawater adaptation. Endocrinol. Jap. 19: 489–493.

    CAS  Google Scholar 

  • Laidley, C.W. and Leatherland, J.F. 1988. Cohort sampling, anaesthesia and stocking-density effects on plasma cortisol, thyroid hormone, metabolite and ion levels in rainbow trout, Salmo gairdneri. Richardson. J. Fish Biol. 33: 73–88.

    Article  CAS  Google Scholar 

  • Langdon, J.S., Thorpe, J.E. and Roberts, R.J. 1984. Effects of cortisol and ACTH on gill Na+/K+-ATPase, SDH and chloride cells in juvenile Atlantic salmon, Salmo salar L. Comp. Biochem. Physiol. 77A: 9–12.

    Article  Google Scholar 

  • Langhorne, P. and Simpson, T.H. 1986. The interrelationship of cortisol, gill (Na+K) ATPase, and homeostasis during the parr-smolt transformation of Atlantic salmon (Salmo salar L.). Gen. Comp. Endocrinol. 61: 203–213.

    Article  PubMed  CAS  Google Scholar 

  • Leray, C., Colin, D.A. and Florentz, A. 1981. Time course of osmotic adaptation and gill energetics of rainbow trout (Salmo gairdneri R.) following abrupt changes in external salinity. J. Comp. Physiol. 144: 175–181.

    Google Scholar 

  • Madsen, S.S. and Naamansen, E.T. 1989. Plasma ionic regulation and gill Na/K-ATPase changes during rapid seawater transfer of yearling rainbow trout (Salmo gairdneri): Time course and seasonal variation. J. Fish Biol. (In press).

  • Maetz, J. 1969. Observations on the role of the pituitary-interrenal axis in the ion regulation of the eel and other teleosts. Gen. Comp. Endocrinol. Suppl. 2: 299–316.

    Article  Google Scholar 

  • Mayer, N. and Maetz, J. 1967. Cortisol, a sodium excreting factor in the eel (Anquilla anquilla L.). Nature, Lond. 214: 1118–1120.

    Article  CAS  Google Scholar 

  • Pickering, A.D., Pottinger, T.G. and Christie, P. 1982. Recovery of the brown trout, Salmo trutta L., from acute handling stress: a time-course study. J. Fish Biol. 20: 229–244.

    Article  Google Scholar 

  • Pickford, G.E., Panag, P.K.T., Weinstein, E., Torretti, J., Hendler, E. and Epstein, F.H. 1970. The response of the hypophysectomized cyprinodont Fundulus heteroclitus, to replacement therapy with cortisol: Effects on blood serum and sodium-potassium activated adenosine triphosphatase in the gills, kidney, and intestinal mucosa. Gen. Comp. Endocrinol. 14: 524–534.

    Article  PubMed  CAS  Google Scholar 

  • Redding, J.M., Schreck, C.B., Birks, E.K. and Ewing, R.D. 1984. Cortisol and its effects on plasma thyroid hormone and electrolyte concentrations in fresh water and during seawater acclimation in yearling coho salmon, Oncorhynchus kisutch. Gen. Comp. Endocrinol. 56: 146–155.

    Article  PubMed  CAS  Google Scholar 

  • Richman, N.H., III and Zaugg, W.S. 1987. Effects of cortisol and growth hormone on osmoregulation in pre- and desmoltified coho salmon (Oncorhynchus kisutch). Gen. Comp. Endocrinol. 65: 189–198.

    Article  PubMed  CAS  Google Scholar 

  • Richman, N.H., III, Nishioka, R.S., Young, G. and Bern, H.A. 1987. Effects of cortisol and growth hormone replacement on osmoregulation in hypophysectomized coho salmon (Oncorhynchus kisutch). Gen. Comp. Endocrinol. 67: 194–201.

    Article  PubMed  CAS  Google Scholar 

  • Scheer, B.T. and Langford, R.W. 1976. Endocrine effects on the cation-dependent ATPase of the gills of European eels (Anquilla anquilla) and efflux of Na. Gen. Comp. Endocrinol. 30: 313–326.

    Article  PubMed  CAS  Google Scholar 

  • Schreck, C.B. 1982. Stress and rearing of salmonids. Aquaculture 28: 241–249.

    Article  Google Scholar 

  • Specker, J.L. 1980. Hormonal and osmoregulatory aspects of smoltification in coho salmon Oncorhynchus kisutch. Ph.D. Thesis, Oregon State University, U.S.A.

    Google Scholar 

  • Specker, J.L. 1982. Interrenal function and smoltification. Aquaculture 28: 59–66.

    Article  CAS  Google Scholar 

  • Strange, R.J. and Schreck, C.B. 1978. Anesthetic and handling stress on survival and cortisol concentration in yearling chinook salmon (Oncorhynchus tshawytscha). J. Fish. Res. Bd. Can. 35: 345–349.

    CAS  Google Scholar 

  • Wedemeyer, G. 1972. Some physiological consequences of handling stress in the juvenile coho salmon (Oncorhynchus kisutch) and steelhead trout (Salmo gairdneri). J. Fish. Res. Bd. Can. 29: 1780–1783.

    CAS  Google Scholar 

  • Young, G. 1986. Cortisol secretion in vitro by the interrenal of coho salmon (Oncorhynchus kisutch) during smoltification: Relationship with plasma thyroxine and plasma cortisol. Gen. Comp. Endocrinol. 63: 191–200.

    Article  PubMed  CAS  Google Scholar 

  • Zaugg, W.S. and McLain, L.R. 1976. Influence of water temperature on gill sodium, potassium-stimulated ATPase activity in juvenile coho salmon (Oncorhynchus kisutch). Comp. Biochem, Physiol. 54A: 419–421.

    Article  CAS  Google Scholar 

  • Zaugg, W.S. and Wagner, H.H. 1973. Gill ATPase activity related to parr-smolt transformation and migration in steelhead trout (Salmo gairdneri) influence of photoperiod and temperature. Comp. Biochem. Physiol. 45B3: 955–965.

    Google Scholar 

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  1. Institute of Biology, Odense University, Campusvej 55, DK-5230, Odense M, Denmark

    Steffen S. Madsen

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Madsen, S.S. Cortisol treatment improves the development of hypoosmoregulatory mechanisms in the euryhaline rainbow trout, Salmo gairdneri . Fish Physiol Biochem 8, 45–52 (1990). https://doi.org/10.1007/BF00004430

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