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2n egg formation in Populus × euramericana (Dode) Guinier

Evidence from nuclear microsatellite analyses

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Abstract

Three synthetic triploid seedlings were derived from a cross between maternal parent Populus × euramericana (Dode) Guinier and Populus nigra var. italica (Moench.) Koehne via embryo rescue by treating female floral buds with colchicine at certain developmental stages before and after pollination, and identified by flow cytometric analyses. A total of 21 microsatellite primers were used to infer the ploidy of gametes forming the hybrids and the underlying meiotic mechanism. The results suggest that 2n eggs originated from the maternal poplar, contributing the extra haploid genome in the triploids. In addition, first division restitution (FDR) 2n eggs were produced in the maternal tree according to the marker genotypes of these triploids. Cytological analyses of megasporogenesis revealed that meiosis of the maternal parent was asynchronous. This cytological evidence suggests that 2n eggs result from FDR after treatment with colchicine, as a portion of megaspore mother cells (MMCs) were within the first division at the post-pollination stage. These results aid in understanding the mechanism of 2n egg formation in poplar and make the utilization of 2n eggs in polyploid poplar breeding more effectively.

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Acknowledgements

The authors would like to thank Dr. Paul Kretchmer at San Francisco Edit for revising the manuscript. This research was supported by the National Natural Science Foundation of China (No. 31070598), the key Project of the Chinese Ministry of Education (No. 109022), and Chinese Chang-Jiang Scholar funds.

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, China

    Xiaojun Xi, Dan Li, Wenting Xu, Liqin Guo, Jinfeng Zhang & Bailian Li

  2. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, 100083, China

    Xiaojun Xi, Dan Li, Wenting Xu, Liqin Guo, Jinfeng Zhang & Bailian Li

  3. The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing Forestry University, Beijing, 100083, China

    Xiaojun Xi, Dan Li, Wenting Xu, Liqin Guo, Jinfeng Zhang & Bailian Li

  4. Research Institute of Fruit and Forestry, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China

    Xiaojun Xi

  5. Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, 27695, USA

    Bailian Li

  6. Beijing Forestry University, Campus Box 118, Beijing, 100083, China

    Jinfeng Zhang

  7. North Carolina State University, 329 Daniels Hall, Campus Box 7112, Raleigh, NC, 27695-7112, USA

    Bailian Li

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  1. Xiaojun Xi
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Correspondence to Jinfeng Zhang or Bailian Li.

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Communicated by A. Brunner

X. Xi and D. Li contributed equally to this work.

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Xi, X., Li, D., Xu, W. et al. 2n egg formation in Populus × euramericana (Dode) Guinier. Tree Genetics & Genomes 8, 1237–1245 (2012). https://doi.org/10.1007/s11295-012-0510-y

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  • DOI: https://doi.org/10.1007/s11295-012-0510-y

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