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In Vitro Multiplication of Phoenix dactylifera (L)

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Abstract

A method of microcloning via somatic embryogenesis was established in date palm (Phoenix dactylifera L) cultivars. The method has potential for commercial application. Embryogenic cultures were initiated from soft primordial tissues of 2-3-year-old female suckers. The system was optimized first for the genotype ’sayar’ and then its applicability was tested to other genotypes. A method of pre-acclimation using pre-acclimation chambers has been devised, which improved hardening survival greatly. More than 80 clones obtained from the genotype ’sayar’ have been shifted to field for agronomic evaluation.

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Abbreviations

2,4-0:

2,4-dichlorophenoxyacetic acid

BAP:

6-benzyl aminopurine

IAA:

indole-3-acetic acid

NAA:

a-naphthalene acetic acid

Kn:

Kinetin

MS:

Murashige

Skoog:

ECI, Embryogenic callus induction

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Authors and Affiliations

  1. Department of Plant Biotechnology, Rajasthan Agricultural University, Bikaner, 334 001, India

    S. C. Bhargava, S. N. Saxena & R. Sharma

Authors
  1. S. C. Bhargava
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  2. S. N. Saxena
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  3. R. Sharma
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Correspondence to S. C. Bhargava.

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Bhargava, S.C., Saxena, S.N. & Sharma, R. In Vitro Multiplication of Phoenix dactylifera (L). J. Plant Biochem. Biotechnol. 12, 43–47 (2003). https://doi.org/10.1007/BF03263158

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  • DOI: https://doi.org/10.1007/BF03263158

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