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TLR and MBL Gene Polymorphisms in Severe Acute Pancreatitis

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Abstract

Background and objective: Mannose-binding lectin (MBL) and toll-like receptor (TLR)-4 gene polymorphisms have been implicated in inflammatory episodes in a number of studies. In view of the inflammatory nature of acute pancreatitis, we aimed to determine the predictive value of two point mutations in the promoter region at position −550 (H/L variants) and −221 (X/Y variants) of the MBL2 gene, and the Asp299Gly and 119C>A polymorphisms of the TLR4 gene on the occurrence of severe acute pancreatitis (SAP).

Methods: The study included 132 patients with SAP, 106 with mild acute pancreatitis (MAP), and 121 healthy volunteers. Genotypes were determined using restriction fragment length polymorphism analysis of PCR products and by allele-specific PCR.

Results: No significant difference in genotype frequency was noted between the patients with acute pancreatitis and controls for any of the gene loci studied. The distributions of the HY/HY, HY/LY, LY/LY, and LY/LX genotypes of MBL2 gene promoter and 119C>A genotype of the TLR4 gene were similar in patients with mild or severe acute pancreatitis. HY/LX genotype frequency was significantly higher in patients with SAP compared with MAP (26% vs 14%; p = 0.028).

Conclusion: Results indicate that the MBL2 HY/LX genotype plays an important role in the determination of disease severity to acute pancreatitis.

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Notes

  1. MBL2 −550H is the wild-type (G) allele, and −550L is the variant (C). Similarly, MBL2 −221X is the wild-type (G) allele, and −221Y is the variant (C).

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Acknowledgments

This work was supported, in part, by grants from Shandong Natural Foundation (grant no. Y2006C20) and the Qingdao City Foundation (No. kzd05).

The authors have no conflicts of interest that are directly relevant to the content of this study.

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Authors and Affiliations

  1. Department of General Surgery, Affiliated Hospital, Qingdao University Medial College, 16 Jiangsu Road, Qingdao, Shandong Province, 266003, China

    DianLiang Zhang, HongMei Zheng & YanBing Zhou

  2. Research Institute of General Surgery, Jinling Hospital, Nanjing University, Nanjing, Jiangsu Province, China

    BaoJun Yu & JieShou Li

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  1. DianLiang Zhang
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  2. HongMei Zheng
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  3. YanBing Zhou
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Correspondence to DianLiang Zhang.

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Zhang, D., Zheng, H., Zhou, Y. et al. TLR and MBL Gene Polymorphisms in Severe Acute Pancreatitis. Mol Diag Ther 12, 45–50 (2008). https://doi.org/10.1007/BF03256267

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