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An orthotopic colon cancer model for studying the B7-H3 antitumor effect in vivo

  • 2005 SSAT Annual Meeting
  • Published:
Journal of Gastrointestinal Surgery Aims and scope

Abstract

We established an orthotopic animal model of colon cancer in mice and applied this model to study the antitumor effects of B7–H3, the newest member of the B7 family of costimulatory molecules. Colon-26 murine colon\ adenocarcinoma cells were inoculated into the cecal subserosum of mice to induce colon tumor growth. The tumor growth rate and the survival time of the mice were observed. A stable B7–H3 transfected Colon-26 cell line was established and the immunogenic effect was investigated. All mice implanted with wild-type tumor cells had tumor growth in the colon and died. The mean survival rate was 23 days. Mice implanted with C26-B7-H3 had a significantly prolonged survival time of 38 days. Our data suggest that B7–H3 exerts an antitumor effect on adenocarcinoma of the colon and may be considered as an adjuvant immunotherapy in the treatment of colon cancers. Our orthotopic animal model of colon cancer in mice could be applied to in vivo experimental studies of colon cancer.

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Authors and Affiliations

  1. Department of Gastroenterology, University of Heidelberg, Heidelberg, Germany

    Catalin M. Lupu M.D., Christoph Eisenbach M.D., Michael A. Kuefner M.D., Wolfgang Stremmel M.D. & Jens Encke M.D.

  2. Department of General Surgery, University of Heidelberg, Heidelberg, Germany

    Jan Schmidt M.D.

  3. Department of Haematology, University of Heidelberg, Heidelberg, Germany

    Alaviana D. Lupu Ph.D.

Authors
  1. Catalin M. Lupu M.D.
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  2. Christoph Eisenbach M.D.
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  3. Michael A. Kuefner M.D.
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  4. Jan Schmidt M.D.
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  5. Alaviana D. Lupu Ph.D.
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  6. Wolfgang Stremmel M.D.
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  7. Jens Encke M.D.
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Corresponding author

Correspondence to Jens Encke M.D..

Additional information

Catalin M Lupu was supported by a grant from the Deutscher Akademischer Austausch Dienst.

These authors contributed equally to the work.

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Lupu, C.M., Eisenbach, C., Kuefner, M.A. et al. An orthotopic colon cancer model for studying the B7-H3 antitumor effect in vivo. J Gastrointest Surg 10, 635–645 (2006). https://doi.org/10.1007/BF03239969

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  • DOI: https://doi.org/10.1007/BF03239969

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