Abstract
The quality of durum wheat (Triticum turgidum ssp.durum) is influenced by polyphenol oxidase (PPO) activity and its corresponding substrates. A saturated molecular-marker linkage map was constructed previously by using a set of recombinant inbred (RI) lines, derived from a cross between durum wheat cultivars Jennah Khetifa and Cham 1. Quantitative trait loci (QTL) for PPO activity in seeds were mapped in this population. PPO activity in seeds of the parents and 110 RI lines was measured spectrophotometrically. The PPO activity of Cham 1 was significantly lower than that of Jennah Khetifa. QTL analysis of these data indicated that most of PPO activity was associated with major loci on the long arm of chromosome 2A. The trait was found to be strongly associated with the SSR markerXgwm312@2A. With this knowledge, marker-assisted selection can be used to select genotypes with lower PPO activity in durum wheat populations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baik BK, Czuchajowska Z, Pomeranz Y, 1995. Discoloration of dough for oriental noodles. Cereal Chem 72: 198–205.
Bernier AM, Howes NK, 1994. Quantification of variation in tyrosinase activity among durum and common wheat cultivar. J Cereal Sci 19: 157–159.
Hessler TG, Thomson MJ, Benscher D, Nachit MM, Sorrells ME, 2002. Association of a lipoxygenase locus,Lpx-B1, with variation in lipoxygenase activity in durum wheat seeds. Crop Sci 42: 1265–1700.
Jimenez M, Dubcovsky J, 1999. Chromosome location of genes affecting polyphenol oxidase activity in common and durum wheat seeds. Plant Breeding 118: 395–398.
Kruger JE, Morgan B, Matsuo RR, Preston KR, 1992. A comparison of methods for the prediction of Cantonese noodle color. Can J Plant Sci 72: 1021–1029.
Lander ES, Green P,Abrahanson J,Barlow A, Daly MJ, Lincoln SE, Newburg L, 1987. Mapmaker: An integrative computer package for constructing primary linkage maps of experimental and natural populations. Genomics 1: 174–181.
Li WL, Faris JD, Chittoor JM, Leach JE, Hulbert SH, Liu DJ, Chen PD, Gill BS, 1999. Genomic mapping of defense response genes in wheat. Theor Appl Genet 98: 226–233.
Mahoney RR, Ramsay M, 1992. A rapid tyrosinase test for detecting contamination of durum wheat. J Cereal Sci 15: 267–270.
Miskelly DM, 1984. Flour components affecting paste and noodle colour. J Sci Food Agric 35: 463–471.
Nachit MM, Elouafi I, Pagnotta MA, El Saleh A, Iacono E, Labhilili M, et al. 2001. Molecular linkage map for an interspecific recombinant inbred population of durum wheat (Triticum turgidum L. var.durum). Theor Appl Genet 102: 177–186.
Nair SK, Tomar SMS, 2001. Genetics of phenol colour reaction of grains and glumes in tetraploid and hexaploid wheats. J Genet Breed 55: 369–373.
Nelson JC, 1997. QGene: Software for marker-based genomic analysis and breeding. Mol Breed 3: 239–245.
Raman R, Raman H, Johnstone K, Lisle C, Smith A, Martin P, Allen H, 2005. Genetic and silico comparative mapping of the polyphenol oxidase gene in bread wheat (Triticum aestivum L.). Funct Integ Genom 6: 185–200.
Simeone R, Pasqualone A, Clodoveo ML, Blanco A, 2002. Genetic mapping of polyphenol oxidase in tetraploid wheat. Cell Mol Biol Lett 7: 763–769.
Souza E, Windes J, Udall J, 1998. Inheritance of polyphenol oxidase reaction in three recombinant inbred populations of wheat. In: Agronomy Abstracts. Amer. Soc. Agron. Madison, WI: 67.
Sun DJ, He ZH, Xia XC, Zhang LP, Morris CF, Appels R, et al. 2005. A novel STS marker for polyphenol oxidase activity in bread wheat. Mol Breed 16: 209–218.
Udall J, 1996. Important alleles for noodle quality in winter wheat as identified by molecular markers. MS Thesis, University of Idaho, Aberdeen, ID, USA.
Watanabe N, Takeuchi A, Nakayama A, 2004. The inheritance and chromosomal location of the genes for phenol colour reaction of grain in durum wheat. Euphytica 139: 87–93.
Wrigley CW, McIntosh RA, 1975. Genetic control of factors regulating the phenol reaction of wheat and rye grain. Wheat Inform Serv 40: 6–10.
Zeven AC, 1972. Identification of chromosomes carrying a locus for a gene conditioning the production of tyrosinase in wheat grains. Wheat Inf Serv 35: 3–8.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Watanabe, N., Akond, A.S.M.G.M. & Nachit, M.M. Genetic mapping of the gene affecting polyphenol oxidase activity in tetraploid durum wheat. J Appl Genet 47, 201–205 (2006). https://doi.org/10.1007/BF03194624
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03194624