Abstract
The interaction of nelin, a cardiac-specific expressed protein of human novel genenelin, with F-actin was studied by both F-actin cosedimentationin vitro and colocalization assays. The results showed that nelin is a new F-actin binding protein and is colocolized with F-actin in cytoplasm of cells. Three new nelin binding proteins, filamin C subtype, titin N2B subtype and inter-alpha trypsin inhibitor heavy chain precursor (ITIH), were identified from human heart cDNA library using yeast two-hybrid screening. The binding activity of filamin C with nelin was confirmed by coimmunoprecipitation. Filamin C binds to nelin through its C-terminal region. It is indicated that nelin is a cytoskeleton associated protein and acts as a membrane-cytoskeleton associated protein involved in the formation of focal adhesions.
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Wang, Z., Meng, X., Cao, H. et al. Characteristics of the binding features of Nelin with F-actin and screening Nelin interactive proteins. Chin.Sci.Bull. 49, 2487–2490 (2004). https://doi.org/10.1007/BF03183719
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DOI: https://doi.org/10.1007/BF03183719